Identification and characterization of five polymorphic microsatellite loci in the freshwater copepod Hemidiaptomus gurneyi (Copepoda: Calanoida: Diaptomidae)

Figures & data

Table I. Polymorphic microsatellite loci scored for Hemidiaptomus gurneyi. Bp: base pairs; Na: number of scored alleles; H_O: observed heterozygosity; H_E: expected heterozygosity.

Locus	Repeat motif	Primer sequence 5ʹ -> 3’	Size range (bp)	Na	Ho	He	Fluorescence label	Multiplex panel
1. Hemgur_000378	(TTAGG)12	F: AGTCAGCGGTTCGCAATTT R: CCGAGCATCTTCTTGTTTCC	154–170	8	0.401	0.399	VIC/1	1
2. Hemgur_001409	(TACA)18	F: TCCGCCGTTGAAGGTTTATC R: GTTCCTATCCCCGACATCCCC	73–159	6	0.171	0.197	PET/1	1
4. Hemgur_201232	(CTAT) 7	F: GATTGCCCCTCTGTTTTCCG R: CACACCTCGTCACACACTTG	139–145	5	0.305	0.313	VIC/3	3
9. Hemgur_205235	(ATG)7	F: TGTCGACGAGAATGGTGATG R: ACTGAGGAAAAGATTGACAGTGC	116–119	3	0.071	0.079	FAM/2	2
10. Hemgur_207140	(ATCT)11	F: GTAGCAAAGTCACGGGTTCG R: AACAGTTCACCTGGCAAAGC	153–166	8	0.511	0.460	FAM/4	4

Table II. List of the sampled localities.

Country	Site name	Population code	Latitude N (WGS84)	Longitude E (WGS84)	Elevation (m a.s.l.)
Tunisia	Oued El Amor	F014	36.927287	8.755628	4
Tunisia	Sejenane	F015	37.056287	9.233482	140
Tunisia	Tabarka	F249	36.936295	8.761124	2
Algeria	Kebouda	T4	36.732700	8.029717	3
Italy	Gorgo del Cerro	PA079	37.889944	13.394575	750

a.s.l.: above sea level.

Table III. Characteristics of the five polymorphic microsatellite loci in five different Hemidiaptomus gurneyi populations. H_O: observed heterozygosity; H_E: expected.

Locus	Population	Specimens	HO	HE	Null allele	PHW
1. Hemgur_000378	F014	16	0.750	0.659	No	1
	F015	20	0.650	0.723	No	0.141
	F249	19	0.526	0.513	No	1
	T4	13	0.231	0.345	No	0.372
	PA079	16	0.250	0.226	No	1
2. Hemgur_001409	F014	16	-	-	No	No test done
	F015	20	0.650	0.711	No	0.512
	F249	20	-	-	No	No test done
	T4	16	0.375	0.502	No	0.328
	PA079	16	-	-	No	No test done
4. Hemgur_201232	F014	16	0.187	0.175	No	1
	F015	20	0.450	0.358	No	0.528
	F249	19	0.05	0.145	No	0.025
	T4	16	0.375	0.643	Yes	0.039
	PA079	20	0.700	0.642	No	0.041
9. Hemgur_205235	F014	16	-	-	No	No test done
	F015	20	0.300	0.261	No	1
	F249	20	-	-	No	No test done
	T4	16	0.125	0.226	No	0.190
	PA079	17	-	-	No	No test done
10. Hemgur_207140	F014	16	0.812	0.669	No	0.387
	F015	19	0.526	0.501	No	1
	F249	20	0.750	0.592	No	0.581
	T4	5	0.625	0.718	No	0.516
	PA079	20	0.350	0.358	No	1

heterozygosity; P_HW: probability that the population is in Hardy Weinberg equilibrium; -: monomorphic.

Table IV. Pairwise comparison among the studied Hemidiaptomus gurneyi populations. Below the diagonal: Fst values; above the diagonal: linear distance between populations (km); *: significant value after Bonferroni correction (α = 0.05).

	F014	F015	F249	T4	PA079
Oued El Amor – F014	-	44.9	1.1	68.3	424.3
Sejenane – F015	0.45677*	-	44.1	113.2	379.5
Tabarka – F249	−0.00609	0.50901*	-	69.1	423.5
Kebouda – T4	0.41813*	0.43387*	0.48276*	-	492.5
Gorgo del Cerro – PA079	0.59689*	0.56698*	0.63524*	0.46733*	-