The need for pure enzymes that degrade cell wall polysaccharides and how to get them

Abstract

Pure, well-characterized, polysaccharide-degrading enzymes are highly desirable for the study of plant cell walls, but until recently have been tedious to prepare. We have expressed polysaccharide-degrading enzymes from fungi in Pichia pastoris, from which they can be purified very easily, and have partially characterized their modes of action.

Keywords:

• Capillary zone electrophoresis
• cell walls
• fungi
• mode of action, Pichia pastoris
• polysaccharide-degrading enzymes

Acknowledgements

This work was supported in part by DOE grant DE-FG02-96ER20215 and has been approved for publication by the Director of the Oklahoma Agricultural Experiment Station. We specially thank M. Pierce for critical review of the manuscript.

Notes

ANTS, aminonaphthalene trisulfonate; CZE, capillary zone electrophoresis.

Additional information

Notes on contributors

AJ Mort

ANTS, aminonaphthalene trisulfonate; CZE, capillary zone electrophoresis.