First Report of Two Colletotrichum Species Associated with Bitter Rot on Apple Fruit in Korea – C. fructicola and C. siamense

Figures & data

Table 1. Information and GenBank accession numbers of Colletotrichum strains isolated this study.

Species	Collection no.	Location	Pathogenicity^a	Accession number
				ITS	ACT	CAL	CHS-1	GAPDH
C. fructicola	CNARE13111	Andong, Gyeongsangbuk-do	+	MG807419	MG831722	MG831728	MG831734	MG831740
	CNARE13132	Andong, Gyeongsangbuk-do	+	MG807420	MG831723	MG831729	MG831735	MG831741
C. siamense	CNARE13100	Andong, Gyeongsangbuk-do	++	MG807421	MG831724	MG831730	MG831736	MG831742
	CNARE13105	Andong, Gyeongsangbuk-do	++	MG807422	MG831725	MG831731	MG831737	MG831743
	CNARE13126	Andong, Gyeongsangbuk-do	+++	MG807423	MG831726	MG831732	MG831738	MG831744
	CNARE13152	Andong, Gyeongsangbuk-do	++	MG807424	MG831727	MG831733	MG831739	MG831745

^a –: no infection and symptom; +: 0.1–6 mm; ++: 7–12 mm; and +++:  12 mm.

Table 2. Comparison of the morphological characteristics of the strain used in this study with those of previously reported C. fructicola and C. siamense.

Characteristic	C. fructicola CNARE13132	C. fructicola^a	C. siamense CNARE13126	C. siamense^a
Colony morphology	Cottony, gray to dark gray at the center	Cottony, gray to dark gray at the center	Cottony, aerial mycelium grayish white, orange conidial masses at the center	Cottony, aerial mycelium white, orange conidial masses at the center
Conidia size (μm)	11.0–14.0 × 4.5–5.5	11.5–17.5 × 3–5.5	11.0–14.5 × 4.0–5.0	12.0–15.5 × 4.0–5.5
Conidia shape	Cylindrical, both ends rounded	Cylindrical, both ends rounded	Cylindrical, both ends bluntly rounded	Cylindrical, both ends bluntly rounded
Colony radius (mm)	71–77	74–79	65–72	79

^a Described by Lee et al. [9].

Figure 1. Maximum likelihood phylogenetic tree based on the concatenated dataset (ITS, ACT, CAL, CHS-1, and GAPDH) used to identify Colletotrichum strains isolated from bitter rot on apple in Korea. Bootstrap scores greater than 50 are presented at the nodes. The scale bar indicates the number of nucleotide substitutions per site and the letter ^Tindicates ex-type strains. The strains originating from bitter rot on apple are indicated in bold.

Figure 2. Colletotrichum fructicola CNARE13132 (a) and C. siamense CNARE13126 (b). (a) Colony morphology on potato dextrose agar (PDA) after 7-day culture at 25 °C (front); (b) Colony morphology on PDA after 7-day cultures at 25 °C (reverse); (c) Conidia Bar scale, 10 μm (micrometer); (d) Symptoms induced by artificial inoculation.

Lee DH, Kim D, Jeon Y, et al. Molecular and cultural characterization of Colletotrichum spp. causing bitter rot of apples in Korea. Plant Pathol J. 2007;23:37–44.

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