Identification and Antifungal Susceptibility Profiles of Cyberlindnera fabianii in Korea

Figures & data

Table 1. Medical history of the patients for the isolates used in this study.

Isolate number	Age/ Sex	Isolated source	Risk factor (Medical history)	Indwelling cathethers	API 20C or VITEK-2	ASTA MALDI-OF (score)
MFCCKHY00016	46/F	Urine	Potine Intracarinal hemorrhage, Antibacterial therapy	Urine foley catheter	C. utilis	Pichia fabianii (146)
MFCCKHY00033	88/M	Urine	Chronic kidney disease, Sepsis, Antibacterial therapy	Urine foley catheter	C. pelliculosa	Pichia fabianii (138)
MFCCKHY00039	52/M	Urine	Subarachnoid hemorrhage from middle cerebral artery	Urine foley catheter	C. pelliculosa	Pichia fabianii (164)
MFCCKHY00043	77/F	Urine	Intertrochanteric fracture of femur, Surgery, Antibacterial therapy	Urine foley catheter	C. pelliculosa	Pichia fabianii (158)
MFCCKHY00045	88/F	Urine	Meningioma of spinal cord, Surgery	Urine foley catheter	C. pelliculosa	Pichia fabianii (151)
MFCCKHY00056	57/M	Urine	Spinal cord injury, Neurogenic bladder	Urine foley catheter	C. pelliculosa	Pichia fabianii (157)
MFCCKHY00084	43/F	Urine	Congestive heart failure, Sepsis, Antibacterial therapy	Urine foley catheter	C. pelliculosa	Pichia fabianii (134)
MFCCKHY00150	51/F	Blood	Pneumoniae, Sepsis, Antibacterial therapy	Urine foley catheter Cervical vein catheter	C. pelliculosa	Pichia fabianii (155)
MFCCKHY00205	72/M	Sputum	Lung metastasis, neutropenia, Antibacterial therapy, empirical antifungal therapy	Chemoport	C. albicans	Pichia fabianii (186)
MFCCKHY00208	82/M	Urine	Multiple fracture of ribs, Antibacterial therapy	Urine foley catheter	C. pelliculosa	Clostridium sporogenes (118)
MFCCKHY00264	75/M	Sputum	MCA territory infection	Urine foley catheter L-tube feeding	C. pelliculosa	Pichia fabianii (146)

Figure 1. Cyberlindnera fabianii. (a) and (b) budding cells, (c) pseudohyphae. Scale bars = 10 μm.

Figure 2. Phylogenetic tree inferred from neighbor-joining analysis of the he D1/D2 region of the large subunit (LSU) sequences from Cyberlindnera fabianii species. The nodal significance was evaluated by means of bootstrapping performed using 1000 replicates.

Table 2 Antifungal susceptibility testing of Cyberlindnera fabianii (n = 11) using Sensititre YeastOne^TM YO10.

Antifungal agents (μg/ml)	0.015	0.03	0.06	0.12	0.25	0.5	1	2	4	8	16	>16
Amphotericin B					5	6
Voriconazole	3	6	1		1
Fluconazole						2	7		1	1
Posaconazole				3	6	1	1
Itraconazole			2	4	4	1
Flucytosine (FC-5)			7	4
Anidulafungin	1		1	9
Micafungin		9	2
Caspofungin		2	9

Figure 3. The comparison of MALDI-TOF profiles of Candida and its related species. The species used in the comparison were Cy. fabianii, C. glabrata, C. krusei, C. pelliculosa, C. tropicalis and C. utilis and MALDI-TOF profiles of each species were presented from left to right. MALDI-TOF profiles mainly between m/z 4000 and 12000 were compared. MicoIDSys MALDI-TOF system was used for this comparison.

Figure 4. The ITS alignment of Cyberlindnera fabianii and NAC strains.