First Report of Gray Mold Disease on Endangered Species Cypripedium japonicum

Figures & data

Figure 1. Symptoms of gray mold disease on C. japonicum. (A, B) Gray mold symptom of C. japonicum at a natural habitat; (C) Colony morphology of B. cinerea on PDA media; (D) Conidia of B. cinerea, isolated from C. japonicum. Red bar presents 50 μm.

Table 1. Comparison of morphological characteristics of gray mold fungus isolated from Cypripedium japonicum.

Characteristics	Isolate in this study	B. cinerea [9]
Colony
Color	Grayish brown	Grayish brown
Conidia
Shape	Ellipsoidal or ovoid	Ellipsoidal or ovoid
Size (μm)	7.1 ∼ 13.4 × 5.2 ∼ 13.4	8 ∼ 16 × 5∼10
Color	Colorless or pale brown	Colorless or pale brown
Conidiophore
Size (μm)	16 ∼ 31 × 1.8 ∼ 2	16 ∼ 31 × 2

Figure 2. Phylogenetic tree obtained through the maximum-likelihood and maximum parsimony analyses using MEGA 10 program base on the (A) ITS, (B) HSP60, and (C) G3PDH sequence of the isolates of B. cinerea and that of other B. cinerea isolates were retrieved from GenBank.

Table 2. List and information of primers for fungal identification.

Target gene	Primer name	Primer sequence 5′-3′	Reference
G3PDH	G3PDHfor G3PDHrev	ATTGACATCGTCGCTGTCAACGA ACCCCACTCGTTGTCGTACCA	Staats et al. [11]
HSP60	HSP60for HSP60rev	CAACAATTGAGATTTGCCCACAAG GATGGATCCAGTGGTACCGAGCAT	Staats et al. [11]
ITS	ITS1 ITS4	TCCGTAGGTGAACCTGCGG TCCTCCGCTTATTGATATGC	Staats et al. [11]

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