Rediscovery of Seven Long-Forgotten Species of Peronospora and Plasmopara (Oomycota)

Figures & data

Table 1. Information of Oomycetes specimens used in this study.

Oomycete species	Host plant	Geographic origin	Herb. No.	GenBank accession No.
				ITS rDNA	cox2 mtDNA	LSU rDNA
Peronospora cheonopodii-ambrosioides	Chenopodium ambrisioides	Korea, Jeju	KUS-F20063	MT734673	MT731360	–
P. cheonopodii-ficifolii	Chenopodium ficifolium	Korea, Namyangju	KUS-F18857	AY211018	MT731363	–
P. clinopodii	Clinopodium cf. vulgare	Korea, Yangpeong	KUS-F20032	MT734670	MT731361	–
P. elsholtziae	Elsholtzia ciliata	Korea, Chuncheon	KUF-F20252	MT734671	MT731362	–
P. lathyrina	Lathyrus japonicus	Korea, Samcheok	KUS-F19431	MT734672	MT731359	–
Plasmopara angustiterminalis	Xanthium strumarium	Korea, Jeongseon	KUS-F24490	–	MT731364	MT29826
Pl. siegesbekiae	Siegesbeckia glabrescens	Korea, Hoengseong	KUS-F21312	–	EU743805	EU743814

Figure 1. Maximum likelihood trees of Plasmopara species based on the LSU ribosomal DNA (D1/D2/D3) sequences (A) and the cox2 mitochondrial DNA sequences (B), with support values in minimum evolution inference. Bootstraping support values (minimum evolution/maximum likelihood) higher than 60% are given above the branches. The scale bar equals the number of nucleotide substitutions per site.

Table 2. Genbank accession numbers of the ITS, 28S rDNA, and cox2 mtDNA sequences used for the phylogenetic study.

Oomycete species	Genbank accession number
	ITS rDNA	28s rDNA	cox2 mtDNA
P. boni-henrici	AY198286	–	DQ365720
P. campestris	AY608609	–	KJ654274
P. chenopodii	EF614955	–	KJ654201
P. chenopodii-polyspermi	AY198291	–	KJ654202
P. chrysosplenii	DQ643839	–	KJ654280
P. corydalis	AY211015	–	KJ654280
P. effusa	DQ643876	–	FJ649411
P. lamii	DQ643902	–	JF527436
P. manshurica	AY286222	–	AY286222
P. rumicis	AY465758	–	KJ654168
P. trigonotidis	AY608611	–	KJ654301
P. vaiabilis	EF614967	–	DQ365734
Pl. angustiterminalis	–	EU743802	EU743812
Pl. baudysii	–	AY250147	–
Pl. chaerophylli	–	AY250163	–
Pl. densa	–	AY035525	DQ365754
Pl. epilobii	–	AY250178	–
Pl. geranii	–	DQ148403	–
Pl. geranii-sylvatici	–	DQ148401	–
Pl. halstedii	–	AY035523	EU743813
Pl. megasperma	–	–	DQ365755
Pl. nivea	–	–	DQ365756
Pl. obducens	–	AY035522	DQ365757
Pl. pimpinellae	–	AY035519	DQ365758
Pl. praetermissa	–	DQ148395	–
Pl. pusilla	–	DQ148402	DQ365759
Pl. sii	–	AY035518	–
Pl. solidaginis	–	AY250144	–
Pl. umbelliferarum	–	AY250161	–
Pl. viticola	–	AY035524	DQ365760
P. belbahrii	KX228833	–	KT828759
P. elshotziae	FJ527442	–	FJ527435
P. swiglei	–	–	FJ394338
P. viciae	MN620384	–	KJ654294
P. lathyrina	EU174896	–	–

Figure 2. Maximum likelihood trees of Peronospora species based on the internal transcribed spacer (ITS) sequences (A) and the cox2 mitochondrial DNA sequences (B), with support values in minimum evolution inference. Bootstraping support values (minimum evolution/maximum likelihood) higher than 60% are given above the branches. The scale bar equals the number of nucleotide substitutions per site.

Figure 3. Morphological characteristics of two Plasmopara species, Pl. angustiterminalis ex Xanthium strumarium (A–E) and Pl. siegesbeckiae ex Siegesbeckia glabrescens (F–J). (A & F), Sporangiophores; (B, C, G, H), Ultimate branchlets; (D, E, I, J), Sporangia (scale bars: 100 µm for sporangiophores, 10 µm for ultimate branchlets, and 10 µm for sporangia).

Figure 4. Morphological characteristics of five Peronospora species, P. chenopodii-ambrosioidis ex Chenopodium ambrosioides (A, F, K, P), P. chenopodii-ficifolii ex Chenopodium ficifolium (B, G, L, Q), P. clinopodii ex Clinopodium cf. vulgare (C, H, M, R), P. elsholtziae ex Elsholtzia ciliata (D, I, N, S), and P. lathyirna ex Lathyrus japonicus (E, J, O, T). (A–E), Conidiophores; (F–J), Ultimate branchlets; (K–T), Conidia (scale bars: 100 µm for conidiophores, 10 µm for ultimate branchlets, and 20 µm for conidia).