Characterization of Soil Microorganism from Humus and Indigenous Microorganism Amendments

Figures & data

Table 1. Microbial population of humus and indigenous microorganisms (IMOs).

Sample	Population (CFU^a) of microorganism (Mean ± SEM^b)
	Fungi × 10^4	Bacteria × 10^6
Humus	5.0 ± 1.5	10.8 ± 2.5
IMO1	7.46 ± 1.7	6.0 ± 2.4
IMO2	2.09 ± 1.11	1.87 ± 1.74
IMO3	7.65 ± 1.34	13.41 ± 2.22
IMO4	4.8 ± 1.9	4.4 ± 0.5

^aCFU: colony forming unit; ^bStandard error of the mean of three replications.

Table 2. The diversity and abundance of different fungal species isolated from humus and IMOs samples.

Fungal genus	Humus	IMOs
Aspergillus	34 ± 11.3	28 ± 9.3
Penicillium	25 ± 8.3	11 ± 3.6
Trichoderma	23 ± 7.6	11 ± 3.6
Rhizopus	15 ± 5	8 ± 2.6
Fusarium	11 ± 3.6	5 ± 1.6
Alternaria	22 ± 7.3	15 ± 5
Botritis	7 ± 2.3	12 ± 4
Unknown	24 ± 8	14 ± 4.6
Total	161	104

Table 3. Biological activities of the isolated bacteria from humus and IMOs.

Strains	Biological activity				Antifungal activity
	Gram staining	Siderophore production	Phosphate solubilization	Starch Hydrolysis	F. oxysporum	S. sclerotium
Pseudomonas aeruginosa (KACC 11085)	+	+	+	+	++^a	++
Bacillus-CCJ74	+	+	+	+	++	+++
Bacillus-CCJ617	+	+	+	+	++	−
Bacillus-CCJ83	+	+	−	−	+	−
Bacillus-CCJ73	+	+	+	−	++	+++
Bacillus-CCJ822	+	+	+	+	++	−
Bacillus-CCJHP43	−	−	−	+	−	−
Pseudomonas-CCJ18	+	+	+	+	++	++
Pseudomonas-CCJ20A	+	+	+	+	+	+
Pseudomonas-CCJ18B	+	+	−	−	+	−
Bacillus-CCJH38	+	+	+	+	+++	+
Bacillus-CCJHP38	−	−	+	−	+	−
Bacillus-CCJHP25	+	+	+	+	−	−
Bacillus-CCJHP381	+	+	+	+	+	+
Bacillus-CCJIMT213	+	−	−	+	−	−
Micrococcus-CCJHP47	±^b	+	±	±	+++	+++
Methylobacterium-CCJHP433	±	±	±	±	++	++

^aInhibition zone of above 1 cm was considered as strong (+++) superscript; around 1 cm was moderate (++), 0.5 cm was good (+) and non inhibition zone was (−), respectively; ^bNot clear to determine.