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Research Articles

Screening of Endophytic Fungal Isolates Against Raffaelea quercus-mongolicae Causing Oak Wilt Disease in Korea

, , & ORCID Icon
Pages 484-494 | Received 11 May 2020, Accepted 21 Sep 2020, Published online: 23 Oct 2020

Figures & data

Table 1. Antifungal isolates of endophytic fungi against R. quercus-mongolicae in the primary screening at 5 days after inoculation in the dark at 25 °C.

Figure 1. Dual culture test results showing mycelial growth inhibition of R. quercus-mongolicae by endophytic fungal isolates with strong antifungal activity at 7 days after inoculation in the dark at 25 °C.

Figure 1. Dual culture test results showing mycelial growth inhibition of R. quercus-mongolicae by endophytic fungal isolates with strong antifungal activity at 7 days after inoculation in the dark at 25 °C.

Table 2. Mycelial growth inhibition rate (%) of endophytic fungal isolates against oak wilt fungus in dual culture test at 7 days after inoculation in the dark at 25 °C.

Figure 2. Mycelial growth inhibition rate (MGI %) of endophytic fungal isolates against oak wilt fungus in culture filtrate test at 5 days after inoculation in the dark at 25 °C. Different letters indicate a significant difference (p < 0.05) among treatments by Tukey’s HSD test.

Figure 2. Mycelial growth inhibition rate (MGI %) of endophytic fungal isolates against oak wilt fungus in culture filtrate test at 5 days after inoculation in the dark at 25 °C. Different letters indicate a significant difference (p < 0.05) among treatments by Tukey’s HSD test.

Figure 3. Mycelial growth of R. quercus-mongolicae on culture media containing unheated or heated culture filtrate of endophytic fungal isolates at 5 days after inoculation in the dark at 25 °C (a: unheated media; b: heated media).

Figure 3. Mycelial growth of R. quercus-mongolicae on culture media containing unheated or heated culture filtrate of endophytic fungal isolates at 5 days after inoculation in the dark at 25 °C (a: unheated media; b: heated media).

Figure 4. Sporulation inhibition rate (SI%) of endophytic fungal isolates against R. quercus-mongolicae in culture filtrate test at 12 days after inoculation (5 days in the dark at 25 °C and 7 days in room temperature condition). Different letters indicate a significant difference (p < 0.05) among treatments by Tukey’s HSD test.

Figure 4. Sporulation inhibition rate (SI%) of endophytic fungal isolates against R. quercus-mongolicae in culture filtrate test at 12 days after inoculation (5 days in the dark at 25 °C and 7 days in room temperature condition). Different letters indicate a significant difference (p < 0.05) among treatments by Tukey’s HSD test.

Figure 5. Spore germination inhibition rate (SGI %) of endophytic fungal isolates against R. quercus-mongolicae in culture filtrate test at 3 days after inoculation in the dark at 25 °C. Different letters indicate a significant difference (p < 0.05) among treatments by Tukey’s HSD test.

Figure 5. Spore germination inhibition rate (SGI %) of endophytic fungal isolates against R. quercus-mongolicae in culture filtrate test at 3 days after inoculation in the dark at 25 °C. Different letters indicate a significant difference (p < 0.05) among treatments by Tukey’s HSD test.

Figure 6. Spore germination of R. quercus-mongolicae on culture media containing unheated or heated culture filtrate of endophytic fungal isolates at 3 days after inoculation in the dark at 25 °C (a: unheated media; b: heated media).

Figure 6. Spore germination of R. quercus-mongolicae on culture media containing unheated or heated culture filtrate of endophytic fungal isolates at 3 days after inoculation in the dark at 25 °C (a: unheated media; b: heated media).