Taxonomic Re-Evaluation of the Genus Fuscoporia in the Republic of Korea Including Three Unrecorded Species

Figures & data

Table 1. List of Fuscoporia specimens and GenBank accessions used in the study.

Species	Specimens	Accession				Locality	Reference	Remarks
		ITS	nrLSU	RPB2	TEF1
Fuscoporia acutimarginata	Dai_16892	MH050752	MH050766	MH079393	MN848822	China	[9]	TYPE
Fuscoporia ambigua	JV_0509/151	MN816707	MN809996	MN848792		USA	[23]	TYPE
Fuscoporia americana	JV_1309/13-J	KJ940023	MG008463		MH636385	USA	[24]	TYPE
Fuscoporia atlantica	SP_445618	KP058515	KP058517			Brazil	[25]	TYPE
Fuscoporia australasica	Dai_15636	MG008397	MG008450	MH079402	MH636408	China	[9]	TYPE
Fuscoporia australiana	Dai_18879	MN816705	MN810015	MN848767	MN848850	Australia	[26]	TYPE
Fuscoporia bambusae	Dai_16599	MN816711	MN809999		MN848808	Thailand	[26]	TYPE
Fuscoporia bambusicola	Cui_8692	MN816739	MT032486		MN848813	China	[26]	TYPE
Fuscoporia beninensis	OAB0132	ON876015				Benin	[13]	TYPE
Fuscoporia callimorpha	Dai_17388	MN121765	MN121824			Brazil	[9]
	Dai_17389	MN121766	MN121825			Brazil	[9]
Fuscoporia caymanensis	JV_1908/74	MT676832	MT676833			French Guiana	[27]	TYPE
Fuscoporia centroamericana	JV_1607/93	MG008444	MG008460		MH636389	Costa Rica	[24]	TYPE
Fuscoporia chinensis	Dai_15713	MN816721	MN810008	MN848771	MN848846	China	[26]	TYPE
Fuscoporia chrysea	JV_1607/106-J	MN816736	MN810027	MN848773	MN848818	Costa Rica	[26]
Fuscoporia contigua	Dai_16025	MG008401	MG008454	MH079406	MH636386	USA	[24]
Fuscoporia costaricana	JV_1407/92	MG008446	MG008461		MH636400	Costa Rica	[24]	TYPE
Fuscoporia discipes	Wei_4947		HQ328524			China	[26]
Fuscoporia dolichoseta	SFC20140723-58	ON427788*	ON427816*	ON479797*	ON479820*	Korea	[18]
	SFC20161006-16	ON427789*	ON427817*	ON479798*	ON479821*	Korea	[18]
	SFC20190731-26	ON427790*	ON427818*	ON479799*		Korea	[18]
	SFC20191015-23	ON427765*	ON427795*	ON464731*		Korea	[18]	TYPE
	SFC20230516-01	OR724641*	OR976466*	OR727960*	OR727969*	Korea	This study
	KUC20121123-07	KJ668545*	OR976469*	OR727961*		Korea	[17]
Fuscoporia eucalypti	Dai_18792	MN816731	MN810022	MN848778	MN848831	Australia	[26]	TYPE
Fuscoporia ferrea	Cui_11801	KX961101	KY189101	MN159387	MN848823	China	[26]
	FP-133592-Sp	KU139189	KU139259	KU139319	KU139379	USA	[28]
	MUCL_45984	KX961112	KY189112		MH636403	France	[26]	Type locality
	SFC20150211-02	ON427768*	ON427797*	ON479779*	ON479805*	Korea	This study
	SFC20150522-09	ON427769*	ON427798*	ON479780*		Korea	This study
	SFC20190322-16	ON427770*	ON427799*	ON479781*	ON479806*	Korea	This study
	SFC20190731-43	ON427771*	ON427800*	ON479782*	ON479807*	Korea	This study
	KUC20121102-35	KJ668546*	KJ668399*			Korea	[17]
Fuscoporia ferruginosa	Dai_13200	MN816702	MN809993	MN848793	MN848802	France	[26]
	JV_1309/4	KX961102	KY189102	MH079405	MH636398	Slovakia	[26]
Fuscoporia gilva	CMW47749	MH599106	MH599129		MT108963	South Africa	[29]
	CMW48145	MH599105	MH599130		MT108962	South Africa	[29]
	JV_0709/75	MN816720	MN810007		MN848852	USA	[26]	Type locality
	JV_1209/65	MN816719	MN810006	MN848768	MN848851	USA	[26]	Type locality
	ASIS24343	KF692068*				Uncertain	Unpublished, Seok et al.
Fuscoporia gilvoides	SFC20150702-23	ON427783*		ON479793*	ON479815*	Korea	[18]
	SFC20160621-12	ON427784*	ON427812*	ON479794*	ON479816*	Korea	[18]
	SFC20160629-33	ON427785*	ON427813*	ON479795*	ON479817*	Korea	[18]
	SFC20180426-12	ON427763*	ON427793*	ON464729*	ON479802*	Korea	[18]	TYPE
	SFC20180905-15	ON427786*	ON427814*	ON479796*	ON479818*	Korea	[18]
	SFC20230613-05	OR724642*	OR976467*	OR727962*	OR727967*	Korea	This study
	SFC20230705-50	OR724643*	OR976468*	OR727963*	OR727968*	Korea	This study
	KMCC04881	MN823149*				Uncertain	Unpublished, Han
	OK552	MN080324*				Uncertain	Unpublished, An et al.
Fuscoporia hainanensis	Dai_16105		ON520809		ON616518	China	[10]	TYPE
Fuscoporia insolita	Spirin_5208	MN816724	MN810016		MN848800	Russia	[26]	TYPE
	Spirin_5251	KJ677113				Russia	[30]
	KUC20121102-22	OR724636*	OR976461*			Korea	This study
Fuscoporia karsteniana	Dai_11403	MN816717	MN810003	MN848795	MN848807	China	[26]	TYPE
	Dai_15717	MN816718	MN810004		MN848805	China	[26]
Fuscoporia koreana	SFC20150625-05	ON427776*	ON427805*	ON479787*	ON479810*	Korea	[18]
	SFC20150625-07	ON427777*	ON427806*	ON479788*	ON479811*	Korea	[18]
	SFC20160726-93	ON427762*	ON427792*	ON464728*	ON479801*	Korea	[18]	TYPE
	SFC20171019-11	ON427778*	ON427807*	ON479789*	ON479812*	Korea	[18]
	SFC20180725-17	ON427779*	ON427808*	ON479790*	ON479813*	Korea	[18]
	SFC20220913-11	OR724640*	OR976465*	OR995755*	OR727966*	Korea	This study
	KUC20121123-27	KJ668544*	KJ668397*			Korea	[17]
	PG	PP065751*				Uncertain	Unpublished, Kim et al.
Fuscoporia latispora	JV_0610/VIIK1	MG008436	MG008469		MH636396	Mexico	[24]	TYPE
Fuscoporia monticola	Dai_11860	MG008406	MG008457		MH636390	China	[24]	TYPE
Fuscoporia palomari	JV_1004/5-J	MN816737				USA	[26]	TYPE
Fuscoporia plumeriae	Dai_18858	MN816712	MN810010	MN848769	MN848843	Australia	[26]	TYPE
Fuscoporia pulviniformis	CMW48060	MH599103	MH599126		MT108961	South Africa	[29]
	CMW48600	MH599102	MH599127		MT108960	South Africa	[29]	TYPE
Fuscoporia punctatiformis	Dai_17443	MH050755	MH050764			Brazil	[9]
Fuscoporia ramulicola	Dai_15723	MH050749	MH050762	MH079398	MN848824	China	[26]	TYPE
	Dai_16155	MH050750	MH050763	MH079399	MN848825	China	[26]
	SFC20160316-07	ON427772*	ON427801*	ON479783*	ON479808*	Korea	This study
	SFC20170524-13	ON427773*	ON427802*	ON479784*	ON479809*	Korea	This study
Fuscoporia reticulata	SFC20121010-19	ON427766*				Korea	[18]
	SFC20160115-16	ON427761*	ON427791*	ON464727*	ON479800*	Korea	[18]	TYPE
Fuscoporia rhabarbarina	Dai_16226	MN816743	MN810035	MN848784	MN848838	China	[26]
	Dai_16550	MN816744	MN810036	MN848785	MN848836	China	[26]
Fuscoporia roseocinerea	JV_1407/84	MN816740	MN810030	MN848774	MN848819	Costa Rica	[26]	Type locality
Fuscoporia rufitincta	JV_0904/142	KJ940030	KX058574			USA	[24]	Type locality
Fuscoporia sarcites	JV_0402/20-K	MZ264225	MZ264218			Venezuela	[15]
Fuscoporia semicephala	SFC20170524-08	ON427764*	ON427794*	ON464730*	ON479803*	Korea	[18]	TYPE
	SFC20170712-20	ON427787*	ON427815*		ON479819*	Korea	[18]
Fuscoporia senex	Dai_15775	MN816746	MN810038	MN848787	MN848834	China	[26]
	Dai_17043	MN816747	MN810039	MN848786	MN848835	China	[26]
	SFC20150812-50	ON427774*	ON427803*	ON479785*	OR995756*	Korea	This study
	SFC20150908-49	ON427775*	ON427804*	ON479786*	OR995757*	Korea	This study
	KUC20110922-13	JX463658*	JX463652*			Korea	[17]
Fuscoporia septiseta	Dai_12820	MG008405	MN810033		MH636394	USA	[26]	TYPE
Fuscoporia setifera	Dai_15706	MH050759	MH050769	MN159391	MN848842	China	[26]
	Dai_15710	MH050758	MH050767	MN159390	MN848841	China	[26]
	KUC20140509-06	OR724637*	OR976462*		OR727964*	Korea	This study
	KUC20140613-42	OR724638*	OR976463*			Korea	This study
	KUC20191011-06B	OR724639*	OR976464*		OR727965*	Korea	This study
Fuscoporia shoreae	Dai_17818	MN816735	MN810026		MN848816	Singapore	[26]	TYPE
Fuscoporia sinica	Dai_15468	MG008412	MG008459		MH636392	China	[24]	TYPE
Fuscoporia sinuosa	Dai_20499	MZ264227	MZ264220			China	[15]	TYPE
Fuscoporia subchrysea	Dai_16201	MN816708	MN809997	MN848796	MN848811	China	[26]	TYPE
Fuscoporia subferrea	Dai_16326	KX961097	KY053472	MH079400	MN848826	China	[26]
	Dai_16327	KX961098	KY053473	MH079401		China	[9]	TYPE
Fuscoporia submurina	Dai_19655	MZ264228	MZ264221			Sri Lanka	[15]	TYPE
	Dai_19656	MZ264230	MZ264223			Sri Lanka	[15]
Fuscoporia torulosa	JV_1312/19-Kout	KX961107	KY189107	MN848780	MH636406	Spain	[26]
	JV_1405/2	KX961106	KY189106	MN848779	MH636405	Czech Republic	[26]
Fuscoporia viticola	He_2123	MN816725	MN810017			USA	[26]
Fuscoporia wahlbergii	JV_1312/20-Kout	MN816727	MG008462			Spain	[26]
Fuscoporia yunnanensis	Cui_8182	MH050756	MN810029	MN848789		China	[9]	Type locality
Phellinidium asiaticum	Wei_5610	KR350571	KC859422			China	[31]	Outgroup
Coniferiporia sulphurascens	FP-134848-SP	MT420687	MT416462		MT470375	USA	[32]	Outgroup
Coniferiporia weirii	FP-134801-SP	MT420698	MT416467	MT386029	MT470382	USA	[32]	Outgroup

Sequences obtained in this study are indicated with bold. Sequences submitted from Korea are indicated with asterisks (*), while some of their localities are uncertain.

Figure 1. Maximum-likelihood tree of Fuscoporia species based on four genetic markers (ITS + nrLSU + RPB2 + TEF1). Bootstrap values >60 are indicated at the nodes. A total of 10 species were identified, including three unrecorded species indicated by green color. Seven species indicated by gray color have been previously reported in Korea. Strains whose sequences were newly obtained in this study are indicated in bold, and sequences submitted from Korea are highlighted in blue.

Figure 2. Macroscopic morphology of unrecorded Fuscoporia species. (A) F. insolita (KUC20121102-22); (B) F. ramulicola (SFC20160316-07); (C) F. setifera (KUC20140509-06) (ba: basidiome; po: pore surface). Scale bar = 1 mm.

Figure 3. Microscopic morphology of unrecorded Fuscoporia species. (A) F. insolita; (B) F. ramulicola; (C) F. setifera (s: basidiospores; b1: basidia; b2: basidioles; c: cystidioles; se: setae; h1: generative hyphae; h2: skeletal hyphae; e: encrusted generative hyphae).

Figure 4. Macroscopic morphology of Fuscoporia species. (A) F. ferrea (SFC20150211-02); (B) F. senex (SFC20150812-50). Scale bar = 1 mm.

Figure 5. Microscopic morphology of Fuscoporia species. (A) F. ferrea; (B) F. senex (s: basidiospores; b1: basidia; b2: basidioles; c: cystidioles; se: setae; h1: generative hyphae; h2: skeletal hyphae; e: encrusted generative hyphae).

Figure 6. Taxonomic key for 10 Korean Fuscoporia species. The branch length does not indicate the genetic distance between species. Four recorded species not supported with sequence evidence (F. contigua, F. ferruginosa, F. gilva, and F. viticola) were excluded.

Chen Q, Dai YC. Two new species of Fuscoporia (Hymenochaetales, Basidiomycota) from southern China based on morphological characters and molecular evidence. Mycokeys. 2019;61:75–89. doi: 10.3897/mycokeys.61.46799.

 PubMedGoogle Scholar

Du P, Chen Q, Vlasák J. Fuscoporia ambigua sp. nov., a new species from America and China. Phytotaxa. 2020;456(2):175–185. doi: 10.11646/phytotaxa.456.2.5.

 Web of Science ®Google Scholar

Chen Q, Wu F, Ji X-H, et al. Phylogeny of the genus Fuscoporia and taxonomic assessment of the F. contigua group. Mycologia. 2019;111(3):423–444. doi: 10.1080/00275514.2019.1570749.

 PubMed Web of Science ®Google Scholar

Pires RM, Motato-Vásquez V, de Gugliotta AM. Fuscoporia atlantica sp. nov., a new polypore from the Brazilian Atlantic Rainforest. Mycotaxon. 2015;130(3):843–855. doi: 10.5248/130.843.

 Web of Science ®Google Scholar

Chen Q, Du P, Vlasák J, et al. Global diversity and phylogeny of Fuscoporia (Hymenochaetales, Basidiomycota). Mycosphere. 2020;11(1):1477–1513. doi: 10.5943/mycosphere/11/1/10.

 Web of Science ®Google Scholar

Olou BA, Langer E, Ryvarden L, et al. New records and barcode sequence data of wood-inhabiting polypores in Benin with notes on their phylogenetic placements and distribution. Fungal Syst Evol. 2023;11(1):11–42. doi: 10.3114/fuse.2023.11.02.

 PubMedGoogle Scholar

Vlasák J, Kout J, Chan Q, et al. Fuscoporia caymanensis sp. nov. (Basidiomycota, Hymenochaetaceae), a new species from tropical America. Phytotaxa. 2020;472(2):1179–3155.

 Web of Science ®Google Scholar

Cho Y, Kim D, Lee Y, et al. Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analyses. IMA Fungus. 2023;14(1):12. doi: 10.1186/s43008-023-00117-6.

 PubMed Web of Science ®Google Scholar

Jang Y, Jang S, Lee J, et al. Diversity of wood-inhabiting polyporoid and corticioid fungi in Odaesan National Park, Korea. Mycobiology. 2016;44(4):217–236. doi: 10.5941/MYCO.2016.44.4.217.

 PubMed Web of Science ®Google Scholar

Brazee NJ. Phylogenetic relationships among species of Phellinus sensu stricto, cause of white trunk rot of hardwoods, from Northern North America. Forests. 2015;6(11):4191–4211. doi: 10.3390/f6114191.

 Web of Science ®Google Scholar

Tchoumi JMT, Coetzee MPA, Rajchenberg M, et al. Poroid Hymenochaetaceae associated with trees showing wood-rot symptoms in the Garden Route National Park of South Africa. Mycologia. 2020;112(4):722–741. doi: 10.1080/00275514.2020.1753160.

 PubMed Web of Science ®Google Scholar

Chen Q, Liu L, Zhang DS, et al. Fuscoporia hainanensis sp. nov. (Hymenochaetales, Basidiomycota), a new member of the F. contigua group. Phytotaxa. 2022;558(3):251–262. doi: 10.11646/phytotaxa.558.3.1.

 Web of Science ®Google Scholar

Spirin V, Vlasák J, Niemelä T. Fuscoporia insolita (Hymenochaetales, Basidiomycota), a new species from Russian Far East. Ann Bot Fenn. 2014;51(6):403–406. doi: 10.5735/085.051.0607.

 Web of Science ®Google Scholar

Wu F, Zhou LW, Vlasák J, et al. Global diversity and systematics of Hymenochaetaceae with poroid hymenophore. Fungal Divers. 2022;113(1):1–192. doi: 10.1007/s13225-021-00496-4.

 Web of Science ®Google Scholar

Zhou LW, Vlasák J, Dai YC. Taxonomy and phylogeny of Phellinidium (Hymenochaetales, Basidiomycota): a redefinition and the segregation of Coniferiporia gen. nov. for forest pathogens. Fungal Biol. 2016;120(8):988–1001. doi: 10.1016/j.funbio.2016.04.008.

 PubMed Web of Science ®Google Scholar

Wang XW, Liu SW, Zhou LW. An updated taxonomic framework of Hymenochaetales (Agaricomycetes, Basidiomycota). Mycosphere. 2023;14(1):452–496. doi: 10.5943/mycosphere/14/1/6.

 Web of Science ®Google Scholar