SSR and SRAP marker-based linkage map of Vitis vinifera L.

Figures & data

Table 1. Forward and reverse primer sequences used in SRAP analysis.

Forward primers	Reverse primers
me1 5′-TGAGTCCAAACCGGATA-3′	em1 5′-GACTGCGTACGAATTAAT-3′
me2 5′-TGAGTCCAAACCGGAGC-3′	em2 5′-GACTGCGTACGAATTTGC-3′
me3 5′-TGAGTCCAAACCGGAAT-3′	em3 5′-GACTGCGTACGAATTGAC-3′
me4 5′-TGAGTCCAAACCGGACC-3′	em4 5′-GACTGCGTACGAATTTGA-3′
me5 5′-TGAGTCCAAACCGGAAG-3′	em5 5′-GACTGCGTACGAATTAAC-3′
me6 5′-TGAGTCCAAACCGGTAG-3′	em6 5′-GACTGCGTACGAATTGCA-3′
me7 5′-TGAGTCCAAACCGGTTG-3′	em7 5′-GACTGCGTACGAATTATG-3′
me8 5′-TGAGTCCAAACCGGTGT-3′	em8 5′-GACTGCGTACGAATTAGC-3′
me9 5′-TGAGTCCAAACCGGTCA-3′	em9 5′-GACTGCGTACGAATTACG-3′
me10 5′-TGAGTCCAAACCGGAGG-3′	em10 5′-GACTGCGTACGAATTTAG-3′
me11 5′-TGAGTCCAAACCGGAGA-3′	em11 5′-GACTGCGTACGAATTTCG-3′
me12 5′-TGAGTCCAAACCGGAAA-3′	em12 5′-GACTGCGTACGAATTGTC-3′
me13 5′-TGAGTCCAAACCGGAAC-3′	em13 5′-GACTGCGTACGAATTGGT-3′
me14 5′-TGAGTCCAAACCGGACA-3′	em14 5′-GACTGCGTACGAATTCAG-3′
me15 5′-TGAGTCCAAACCGGACG-3′	em15 5′-GACTGCGTACGAATTCTG-3′
me16 5′-TGAGTCCAAACCGGACT-3′	em16 5′-GACTGCGTACGAATTCGG-3′
me17 5′-TGAGTCCAAACCGGCAT-3′	em17 5′-GACTGCGTACGAATTCCA-3′
me18 5′-TGAGTCCAAACCGGGAC-3′	em18 5′-GACTGCGTACGAATTGAT-3′
me19 5′-TGAGTCCAAACCGGGTA-3′	em19 5′-GACTGCGTACGAATTCAA-3′
me20 5′-TGAGTCCAAACCGGGGT-3′	em20 5′-GACTGCGTACGAATTCAT-3′
me21 5′-TGAGTCCAAACCGGCAG-3′	em21 5′-GACTGCGTACGAATTCTA-3′

Table 2. Distribution of segregation types in the ‘87-1’ × ‘9-22’ population.

‘87-1’		‘9-22’		‘87-1’ and ‘9-22’
1:1		1:1		1:1:1:1		1:1:1:1	1:2:1	Total
lm x ll (codominant)	lm x ll (dominant)	nn × np (dominant)	nn × np (codominant)	ab × cd	ef × eg	hk × hk (codominant)	hk × hk (dominant)	3:1
25	53	27	47	17	40	4	39	252

Table 3. Main characteristics of linkage groups in the integrated, the maternal ‘87-1’ and the paternal ‘9-22’ map.

	Consensus map			Map of female parent ‘87-1’			Map of male parent ‘9-22’
LGs	Covered length (cM)	No. of markers	Gaps (>20 cM) number	Covered length (cM)	No. of markers	Gaps (>20 cM) number	Covered length (cM)	No. of markers	Gaps (>20 cM) number
1	51.7	8	0	46.3	3	1	51.7	7	0
2	19.2	3	0	19.2	3	0	12.4	2
3	139.7	17	3	99.6	13	2	105.6	13	2
4	94.3	9	2	133.1	12	2	69.4	8	1
	63.4	5	1
5	90.7	10	1	90.7	9	1	54.5	6	1
6	115	14	2	47.8	15	0	115	14	2
7	90.6	13	1	34.9	3	1	91.6	11	2
8	74.9	7	1	48.1	5	0	74.9	7	1
8 associated	16.2	4	0	12.8	3		15.6	3	0
9	137.9	17	2	95.6	10	2	153.6	11	4
10	62.4	15	1	94.5	11	1	62.8	13	1
11	9.7	2	0	9.7	2	0	–	–	–
12	79.2	16	0	97.1	10	1	84.7	13	0
13	40.7	5	1	28.7	2	1	40.7	5	1
13 associated	53.9	3	1	53.9	3	1	–	–	–
14	61.7	15	0	61.5	14	0	66	10	0
15	16.2	4	0	16.2	4	0	11	3	0
16	42	12	0	40.6	11	0	39.3	9	1
17	62.4	8	1	62.7	7	1	34.6	4	1
18	108	12	2	77.9	8	2	104.9	6	2
19	107.3	18	0	102	15	0	79.1	13	0
Total	1537.1	217	19	1272.9	163	16	1267.4	158	19
Average	69.8			60.6			63.3