Radioprotective effect of Rapana thomasiana hemocyanin in gamma induced acute radiation syndrome

Figures & data

Table 1. Experimental design of the study.

Experimental groups	RtH (mg/kg)
Non-irradiated mice
Group I	0
Group II	50
Group III	100
Group IV	150
Group V	200
Irradiated mice
Group VI	0
Group VII	50
Group VIII	100
Group IX	150
Group X	200

Table 2. Effect of RtH on the survival rate of experimental mice supplemented with different doses of RtH for five consecutive days before exposure to gamma irradiation with a dose of 7.5 Gy. The experimental groups are as described in Table 1.

		Mortality on different days post-treatment
Groups	RtH (mg/kg b. w.)	5	10	15	20	25	30	Mortality (%)	Survivors/total
Non-irradiated mice
I	0							0%	10/10
II	50							0%	10/10
III	100							0%	10/10
IV	150							0%	10/10
V	200							0%	10/10
Irradiated mice
VI	0		3	7				100%	0/10
VII	50			5	3			80%	2/10
VIII	100			5	2			70%	3/10
IX	150		3			1		40%	6/10
X	200			5				50%	5/10

Figure 1. Spleen colony assay on day 11 post-irradiation. The experimental groups are designated as described in Table 1.

Figure 2. Survival curves of mice supplemented with different doses of RtH: 50 mg/kg (A), 100 mg/kg (B), 150 mg/kg (C) and 200 mg/kg (D). The experimental groups are designated as described in Table 1. Mice were irradiated with 7.5 Gy gamma radiation. Survival was monitored up to 30 days post-irradiation. Statistically significant difference between Group VI (irradiated only), and irradiated Groups supplemented with RtH ( p < 0.05).

Figure 3. Histological haematoxylin/eosin (HE) analysis of experimental gastric ulcers induced by gamma irradiation. Stomach of healthy mice (A), HE × 10; nerve endings in the underlying tissue of the stomach in healthy mice (B), HE × 40; ulceration on the 4th hour after irradiation (C) – noduli lymphatici (thin arrow) and dilated vessel (thick arrow), HE × 40; and vacuolization (D) of the stomach epithelium (thin arrow) and area of normal epithelium (thick arrow), HE × 40; stomach integrity on the 12th hour (E) and 24th hour (F) after radiation exposure; HE × 40.

Figure 4. Stomach ulcer area (mm²) of mice 4–24 h after irradiation exposure. The experimental groups are designated as described in Table 1. Statistical significance: *** p < 0.001 vs. Group I; +++ p < 0.001 vs. Group VI; ++ p < 0.01 vs. Group VI; n.s. – non-significant.

Figure 5. Antioxidant activity (AOA) of RtH and BHT in liposomal syspension. Data are expressed as mean ± SE.