Protein analysis of boar seminal plasma proteins with protective effect during low-temperature storage of spermatozoa

Figures & data

Table 1. Sperm motility after low-temperature storage.

Samples	Progressive motile (%)	Non-progressive motile (%)	Static (%)
C+	43.70 ± 9.49^а	40.75 ± 5.70^a	15.55 ± 4.05^c
C−	14.63 ± 2.06^c	26.31 ± 4.29^c	59.08 ± 2.32^а
Fraction 1	31.60 ± 7.18^b	42.63 ± 4.01^c	25.77 ± 4.09^b
Fraction 2	28.76 ± 8.51^b	39.01 ± 3.06^c	32.23 ± 5.04
Fraction 3	30.32 ± 5.53^b	39.20 ± 8.28	30.48 ± 10.49
Fraction 4	33.28 ± 5.62	32.1 ± 5.77	34.65 ± 11.09
Fraction 5	39.73 ± 11.72	40.54 ± 6.17	19.73 ± 4.10^c
Fraction 6	51.00 ± 5.41^а	32.05 ± 2.11^a	17.95 ± 3.64^c
Fraction 7	37.25 ± 6.92	41.18 ± 6.70	21.57 ± 1.34^b

Note: Significant differences shown between ^a and ^b at P < 0.05; between ^a and ^c at P < 0.001.

Table 2. Sperm velocity parameters (VCL, VSL and VAP).

Samples	VCL (μm/s)	VSL (μm/s)	VAP (μm/s)
C+	46.56 ± 5.39^а	19.10 ± 8.24	31.65 ± 9.48^а
C−	14.63 ± 2.84^c	8.63 ± 1.29^b	13.63 ± 1.69^b
Fraction 1	39.96 ± 12.64	12.73 ± 3.35	18.60 ± 3.78
Fraction 2	34.90 ± 2.25	18.61 ± 4.57	25.69 ± 4.97
Fraction 3	36.87 ± 11.18	12.30 ± 2.11	21.85 ± 5.42
Fraction 4	39.96 ± 6.90	15.25 ± 2.47	26.08 ± 4.77
Fraction 5	33.93 ± 3.55	11.70 ± 3.01^б	19.40 ± 3.11
Fraction 6	47.25 ± 4.64^а	26.75 ± 2.16^а	23.85 ± 3.10
Fraction 7	27.35 ± 6.33	13.00 ± 1.90^b	21.88 ± 2.40

Note: Significant differences shown between ^a and ^b at P < 0.05; between ^a and ^c at P < 0.001.

Figure 1. Representative CFDA/PI double staining of boar spermatozoa incubated at 4 °C for 24 h, with different fractions of seminal plasma proteins.

Figure 2. Silver-stained 2D electrophoresis of fraction 6 isolated from boar seminal plasma by gel permeation chromatography.