Antibacterial potential of streptomycete strains from Antarctic soils

Figures & data

Table 1. Antibacterial activity of Antarctic streptomycetes.

	Antibacterial activity (inhibition zone, mm)
	Streptomycete strains
Test-bacteria	2M	5M	6M	7M	9M	10M	13M	39	2	4	6	8	10–4	11
Bacillus subtilis ATCC^1 6633	27	–	18	33	–	–	40	10	24	34	29	30	37	10
Clavibacter michiganensis NBIMCC^2 2425	38	13	26	36	9	10	43	–	–	–	27	25	23	13
Burkholderia cepacia 23 (onion isolate)^3	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Burkholderia gladioli 31(onion isolate)^3	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Burkholderia pyrrocinia 27 (onion isolate)^3	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Erwinia amylowora NBIMCC 8488 (pear isolate)	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Escherichia coli NBIMCC 3397	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Xanthomonas euvesicatoria 11t (tomato isolate)^3	21	–	15	25	–	–	32	–	–	–	–	11	–	–
Xanthomonas euvesicatoria 52b (pepper isolate)^3	17	–	–	18	–	–	40	–	–	–	11	12	–	–
Xanthomonas gardneri NBIMCC 8730	13	–	15	25	–	–	20	–	–	–	–	–	–	–
Xanthomonas perforans NBIMCC 8729	17	–	18	–	–	–	30	–	–	–	–	–	–	–
Xanthomonas vesicatoria 21t (tomato isolate)^3	–	–	15	15	–	–	20	–	–	–	–	–	–	–

Note: ¹ATCC – American Type Culture Collection; ²NBIMCC – National Bank for Industrial Microorganisms and Cell Cultures (Bulgaria); ³The strains were kindly provided by prof. N. Bogatzevska (Institute of Soil Science, Agrotechnologies and Plant Protection ‘N. Poushkarov’, Sofia, Bulgaria).

Figure 1. PCR-amplification of actinomycetes DNA with the genus-specific primer pair StrepB/StrepE. M- DNA Ladder (Fermentas); 155 – S. hygroscopicus 155; the number of each lane corresponds to the numbers of the strains.

Figure 2. PCR-amplification of actinomycetes DNA with the genus-specific primer pair StrepB/StrepF. M- DNA Ladder (Fermentas); 155 - S. hygroscopicus 155; the number of each lane corresponds to the numbers of the strains.

Figure 3. Phylogenetic tree showing the relation of strain 1M to other Streptomyces species based on the comparative analysis of the sequence of their 16S rDNA gene.

Figure 4. PCR-amplification of streptomycetes DNA for the gene involved in the synthesis of NRPS. M- DNA Ladder (Fermentas); the number of each lane corresponds to the numbers of the strains.

Figure 5. PCR-amplification of streptomycetes DNA for the genes involved in the synthesis of PKS-II. M- DNA Ladder (Fermentas); 155 - S. hygroscopicus 155; the number of each lane corresponds to the numbers of the strains.

Figure 6. PCR-amplification of streptomycetes DNA for glycopeptide antibiotic genes. M- DNA Ladder (Fermentas); the number of each lane corresponds to the numbers of the strains.

Figure 7. PCR-amplification of streptomycetes DNA for the gene involved in the synthesis of polyene antibiotics. M- DNA Ladder (Fermentas); the number of each lane corresponds to the numbers of the strains.