Comparative study of cytotoxicity of detonation nanodiamond particles with an osteosarcoma cell line and primary mesenchymal stem cells

Figures & data

Figure 1. Water suspensions of the studied detonation nanodiamonds with concentration 0.1 mg/ml.

Figure 2. Size distribution of DND particles.

Table 1. ICP-MS analysis of DND samples.

	1	2	3	4
Sample no.	NSFPA	NASHCl	YTM	DND-30
B11 (LR)	21.41	5.87	6.38	15.65
Sr88 (LR)	0.33	1.08	9.13	39.93
Nb93 (LR)	0.37	1.22	4.93	0.4
Mo95 (LR)	1.18	4.56	44.32	5.64
Sn118 (LR)	0.09	1.27	36.86	81.02
Sb121 (LR)	26.58	72.91	0.39	1.48
Ba137 (LR)	176.8	261.36	49.86	3324.31
W182 (LR)	0.25	1.4	4.04	1.82
Pb208 (LR)	97.62	84.79	11.33	1475.11
Bi209 (LR)	0.02	0.03	0.13	0.01
Na23 (MR)	31.6	50.28	243.51	89.93
Mg24 (MR)	2.48	12.84	264.01	1.8
Al27 (MR)	12.71	65.86	459.73	28.94
Si28 (MR)	47.37	148.64	1427.4	319.36
P31 (MR)	0.18	1.39	3.46	2.85
S32 (MR)	388.4	467.9	763.62	2263.66
Ca42 (MR)	0	45.3	3013.76	36.19
Ti47 (MR)	396.01	1217.02	1281.43	28.32
V51 (MR)	5.13	13.64	27.68	0.85
Cr52 (MR)	5.76	4.78	5107.28	9.51
Mn55 (MR)	0.19	1.65	49.34	17.56
Fe56 (MR)	37.16	109.56	328.51	358.6
Ni60 (MR)	9.32	30.71	3.36	21.09
Cu63 (MR)	0.59	14.11	74.88	1.75
Zn66 (MR)	0	161.31	61.88	4.41
K39 (HR)	11.36	18.64	207.49	9.69

Figure 3. Proliferation activity of MG-63 cells (A) and rMSCs (B), incubated for three days with different DND particles.

Figure 4. Overall morphology of MG-63 (A) and rMSCs (B) cells, incubated for three days with different DND particles, bar = 100 μm.