The oxidative stress response of the filamentous yeast Trichosporon cutaneum R57 to copper, cadmium and chromium exposure

Figures & data

Figure 1. Growth of T. cutaneum R57 cells in the presence of different concentrations of metal ions (A) Cu, (B) Cr, and (C) Cd.

Table 1. Increase in ROS generation in the intact cells of T. cutaneum R57 treated by enhanced concentrations of Cu, Cr and Cd ions.

	Cu		Cr		Cd
Variants	^·O2^− (nmol /mg d.w./h)	H2O2 (μmol /L.mg d.w./h)	^·O2^− (nmol/mg d.w./h)	H2O2 (μmol/L.mg d.w./h)	^·O2^− (nmol/mg d.w./h)	H2O2 (μmol/L.mg d.w./h)
Control	0.63	13.1	0.60	15.20	0.69	14.00
0.5 mmol/L	0.65	13.1	Nd	Nd	Nd	Nd
1 mmol/L	0.89	22.4	0.90	33.9	0.86	20.80
3 mmol/L	1.15	21.6	Nd	Nd	Nd	Nd
5 mmol/L	Nd	Nd	1.58	37.5	1.20	22.00
10 mmol/L	Nd	Nd	1.80	28.6	2.41	25.6

Figure 2. Protein oxidation induced in T. cutaneum R57 cells by Cu (A), Cr (B) and Cd (C) ions.

Figure 3. Changes in the glycogen (-o-) and trehalose (-▪-) level in T. cutaneum R57 cells treated with Cu (A), Cr (B) and Cd (C) ions.

Figure 4. Antioxidant response of T. cutaneum R57 against metal-induced stress (A) Cu, (B) Cr, and (C) Cd.