Proteolytic and antimicrobial activity of lactic acid bacteria grown in goat milk

Figures & data

Table 1. Strains and starter cultures with antimicrobial activity.

	Test-microorganisms (active strains)
	E. coli	S. aureus	S. choleraesuis ser. enteritidis	S. choleraesuis ser. typhymurium	L. monocytogenes	E. aerogenes
L. delbrueckii subsp. bulgaricus	(1) b24	(1) b144	(1) b5	(3) b7, b41, b48	0	(19) b144, b24, b38, b21, b5, b7, b2, b28, Db
L. helveticus						Q40, H25, h3P14, H9
L. lactis						1043
S. thermophilus		t2, t18, t12	t2		t2, t24	t2
Starter cultures	LBB BY 144-12, LBB BY 5-12	LBB BY 24, LBB BY 21, LBB BY 5-12, LBB BY 41-8		LBB BY 24, LBB BY 2, LBB BY 21, LBB BY 5-12, LBB BY 41-8, LBB C 216-40

Figure 1. Antibacterial activity of selected LAB grown in goat milk against E. aerogenes ATCC 13048. b21, b24 and b38 – strains of L. delbrueckii subsp. bulgaricus; 3P14 (3П14) – L. helveticus strain.

Figure 2. Proteolytic activity of selected LAB strains cultivated in goat milk. b38, b122, b24, b2, b48, b7 and b21 – strains of L. delbrueckii subsp. bulgaricus; 1043 – strain of L. delbrueckii subsp. lactis; h25, 3P14 (3П14) and Q40 – strains of L. helveticus; 3D1, Dt1, t39, t38, t4, t24, t41 and t21 – strains of S. thermophilus; 1598, 1310, 199, 1588 and 1400 – L. lactis strains.

Figure 3. SDS-PAGE analysis of the whey fraction of goat milk after cultivation of selected LAB and starter cultures. Lane 1: ultra low molecular weight marker (Sigma-Aldrich); Lane 2: high molecular protein marker (Sigma-Aldrich); Lane 3: goat milk; Lane 4: starter culture LBB BY 21; Lane 5: L. delbrueckii subsp. bulgaricus b21; Lane 6: S. thermophilus t21; Lane 7: starter culture LBB YC-1; Lane 8: L. helveticus h3P14; Lane 9: S. thermophilus t3D1; Lane 10: L. helveticus h9.

Figure 4. SDS-PAGE analysis of the whey fraction of goat milk after cultivation of selected LAB and starter cultures. Lane 1: ultra-low-molecular-weight marker (Sigma-Aldrich); Lane 2: high-molecular protein marker (Sigma-Aldrich); Lane 3: goat milk; Lane 4: starter culture LBB C-2; Lane 5: L. delbrueckii subs. bulgaricus b38; Lane 6: S. thermophilus t37; Lane 7: L. helveticus Q40; Lane 8: S. thermophilus tD1; Lane 9: starter culture LBB BY 145-18; Lane 10: L. delbrueckii subsp. bulgaricus b48.