Rapid identification of Pseudomonas aeruginosa by pulsed-field gel electrophoresis

Figures & data

Table 1. Major characteristics, antibiotic susceptibility and biochemical profiles observed in Pseudomonas aeruginosa clinical isolates.

			Zone of inhibition (cm)
Isolate no.	Source	Antibiotype	CAZ	IPM	ATM	CIP	Pigment production	Haemolysis
1	Wound	d	0	3	2.2	3	Yellow	β
2	Wound	d	0	3.2	2.2	2.8	Yellow	β
3	Wound	d	0	3	1.5	2.5	Yellow	β
4	Wound	d	0	3.9	1.7	2.8	Blue–green	β
5	Urine	d	0	2.5	3	2	None	α
6	Wound	b	0	2.3	0	0	Yellow	β
7	Wound	d	0	2.7	2.8	3.2	Blue–green	β
8	Wound	d	0	2.8	1.8	2.7	Yellow	β
9	Urine	d	0	3.6	2.4	2.7	Rosy-brown	β
10	Wound	d	0	2.8	2.6	3.2	Yellow	β
11	Wound	c	0	4.6	0	4.2	None	β
12	Wound	a	1.7	3	2	3	None	β
13	Urine	d	0	2.7	2.8	3.2	No growth	α
14	Wound	d	0	3	1.8	2.5	Blue–green	β
15	Wound	d	0	1.7	2	2.5	No growth	β
16	ATCC 9027	d	0	2.7	2.1	2.5	Yellowish-green	–
17	Wound	d	0	3.4	2	3.6	Pale-yellow	β
18	Wound	d	0	3.4	2.4	2.9	Yellow	β
19	Wound	d	0	3.4	2.5	3	Yellowish-green	β
20	Wound	d	0	3	2.4	2.4	Yellowish-green	β
21	Wound	d	0	3.3	2.8	2.9	Yellowish-green	β

Note: CAZ – ceftazidime (30 μg), IPM – imipenem (10 μg), ATM – aztreonam (30 μg), CIP – ciprofloxacin (30 μg); β – complete blood haemolysis, α – partial blood haemolysis. Antibiotype ‘a’ (fully susceptible to antibiotics), ‘b’ (multiresistant to three types of antibiotics), ‘c’ (multiresistant to two types of antibiotics) and ‘d’ (susceptible to three types of antibiotics with clear zone diameter ranging between 1.5 and 3.5 cm).

Figure 1. DNA genomic patterns of Pseudomonas aeruginosa digested with SpeI. PFGE patterns A, A1, B, B1, C, C1, D, D1, E and F are shown.

Note: L: DNA ladder; Lanes 1–21: isolates (see Table 1); Lane 16 – P. aeruginosa ATCC 9027 control strain.