Genetic diversity assessment of Vitis ficifolia Bge. populations from Henan province of China by SRAP markers

Figures & data

Table 1. Different locations of sample collection in Henan Province of China.

Population code	Locality	Sample size	Longitude E	Latitude N
SH	Shihe, Xinyang	29	113° 90′	31° 96′
NZ	Nanzhao County, Nanyang	25	111° 98′	33° 49′
LN	Luoning, Luoyang	24	111° 48′	34° 35′
XA	Xinan, Luoyang	26	112° 12′	34° 83′
LZ	Linzhou, Anyang	22	113° 75′	36° 07′

Figure 1. Map showing locations of the sampled populations of V. ficifolia Bge.

Table 2. Polymorphism revealed by different primer combinations used in SRAP analysis.

Primer combinations	TNB^a	NPB^b	PPB^c (%)	Size (bp)
Me1/Em6	18	14	77.77	50–1500
Me1/Em8	21	18	85.71	50–1500
Me2/Em8	22	19	86.36	50–1500
Me3/Em7	18	15	83.33	50–1200
Me3/Em8	20	17	85.00	50–1100
Me4/Em6	17	15	88.24	50–1500
Me5/Em6	23	19	82.61	100–1500
Me9/Em6	22	15	68.18	50–1000
Me9/Em13	16	13	81.25	50–800
Me10/Em6	27	22	81.48	100–1000
Me10/Em8	27	16	59.26	150–1500
Me10/Em13	8	6	75.00	150–900
Me11/Em6	13	11	84.62	110–1000
Me11/Em7	13	11	84.62	70–1500
Me11/Em8	20	17	85.00	50–1200
Me12/Em1	15	12	80.00	50–1200
Me13/Em7	20	15	75.00	100–1400
Me13/Em13	15	13	86.67	50–700
Mean	18.6	14.9	80.56
Total	335	268

Notes:

^aTNB = number of total bands.

^bNPB = number of polymorphic bands.

^cPPB = percentage of polymorphic bands.

Table 3. Genetic diversity of different populations.

Population code	NPB^a	PPB^b (%)	H^c	I^d
LZ	187	55.82	0.1112	0.1727
XA	227	67.76	0.1617	0.2335
LN	211	62.99	0.1792	0.2453
NZ	258	77.01	0.1963	0.2689
SH	259	77.31	0.1987	0.2805
Mean	228	68.18	0.1694	0.2402
Species level	268	80.56	0.2129	0.3075

Notes;

^aNPB = number of polymorphic bands.

^bPPB = percentage of polymorphic bands.

^cH = Nei's gene diversity.

^dI = Shannon's information index.

Table 4. Genetic identity and genetic distance of the five studied populations.

Population code	SH	NZ	LN	XA	LZ
SH	–	0.9641	0.9556	0.9353	0.9171
NZ	0.0368	–	0.9729	0.9699	0.9512
LN	0.0455	0.0275	–	0.9825	0.9650
XA	0.0669	0.0305	0.0176	–	0.9676
LZ	0.0866	0.0501	0.0356	0.0329	–

Note: Nei′s genetic identity (above diagonal) and genetic distance (below diagonal).

Figure 2. Dendrogram of the five geographical populations using UPGMA.