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Article; Agriculture and Environmental Biotechnology

Induction of resistance to potato virus Y strain NTN in potato plants through RNAi

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Pages 21-26 | Received 20 Mar 2014, Accepted 29 Oct 2014, Published online: 28 Nov 2014

Figures & data

Figure 1. Concentric necrotic rings on potato tubers.

Figure 1. Concentric necrotic rings on potato tubers.

Figure 2. dsRNAs of the HC-Pro gene region of PVY and S segment of Phi6. (1) 100 bp DNA ladder (100/1000 bp); (2) dsRNAs of the HC-Pro gene region of PVY 1445 bp and (3) dsRNAs of S segment of Phi6 2948 bp.

Figure 2. dsRNAs of the HC-Pro gene region of PVY and S segment of Phi6. (1) 100 bp DNA ladder (100/1000 bp); (2) dsRNAs of the HC-Pro gene region of PVY 1445 bp and (3) dsRNAs of S segment of Phi6 2948 bp.

Figure 3. DAS-ELISA of potato plants after treatment with dsRNA and siRNAs. OD values of samples from: 1/3 – old leaves (at the moment of treatment and virus inoculation) of potato plants treated with HC-Pro dsRNAs and inoculated with PVY; 4/6 – new leaves (leaves grown after treatment) of potato plants treated with HC-Pro dsRNAs and inoculated with PVY; 7/9 – old leaves and 10/12 – new leaves of potato plants treated with HC-Pro siRNAs and inoculated with PVY; 13/15 – old leaves and 16/18 – new leaves of potato plants treated with control dsRNAs from the S segment of Phi 6 and inoculated with PVY; 19/21 – old leaves and 22/24 – new leaves of potato plants treated with control siRNAs from the S segment of Phi 6 and inoculated with PVY; 25/27 – control leaf samples of healthy potato plants not treated and not inoculated with virus; 28/30 – control leaf samples of potato plants inoculated with PVY and not treated; 31/33 – control leaf samples of healthy potato plants treated only with water; 34 – K+ control from the Kit; 35 – K− control from the Kit; 36 – buffer control.

Figure 3. DAS-ELISA of potato plants after treatment with dsRNA and siRNAs. OD values of samples from: 1/3 – old leaves (at the moment of treatment and virus inoculation) of potato plants treated with HC-Pro dsRNAs and inoculated with PVY; 4/6 – new leaves (leaves grown after treatment) of potato plants treated with HC-Pro dsRNAs and inoculated with PVY; 7/9 – old leaves and 10/12 – new leaves of potato plants treated with HC-Pro siRNAs and inoculated with PVY; 13/15 – old leaves and 16/18 – new leaves of potato plants treated with control dsRNAs from the S segment of Phi 6 and inoculated with PVY; 19/21 – old leaves and 22/24 – new leaves of potato plants treated with control siRNAs from the S segment of Phi 6 and inoculated with PVY; 25/27 – control leaf samples of healthy potato plants not treated and not inoculated with virus; 28/30 – control leaf samples of potato plants inoculated with PVY and not treated; 31/33 – control leaf samples of healthy potato plants treated only with water; 34 – K+ control from the Kit; 35 – K− control from the Kit; 36 – buffer control.