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Article; Agriculture and Environmental Biotechnology

Isolation and cDNA characteristics of MHC-DRA genes from gayal (Bos frontalis) and gaytle (Bos frontalis × Bos taurus)

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Pages 33-39 | Received 10 Mar 2014, Accepted 13 Oct 2014, Published online: 16 Dec 2014

Figures & data

Figure 1. RT-PCR result for the DRA genes from gayal (1) and gaytle (2). M: DL2000 DNA marker.

Figure 1. RT-PCR result for the DRA genes from gayal (1) and gaytle (2). M: DL2000 DNA marker.

Table 1. Sequence similarity comparison at the nucleotides and amino acids (within parentheses) sections for the signal peptide (SP), α1, α2 and connecting peptide/transmembrane/cytoplasmatic (CP/TM/CY) domains of the DRA genes from different species compared to those for gayal (B. frontalis, up) and gaytle (B. frontalis × B. taurus, down).

Figure 2. Alignment of the protein encoded by the DRA genes in gayal (Bos frontalis), gaytle (B. frontalis × B. taurus), European cattle (B. taurus, D37956), zebu cattle (Bos indicus, FM986338), yak (Bos grunniens, JQ911700), Chinese yakow (B. grunniens × B. taurus, JQ347519), buffaloes (Bubalus bubalis, DQ016629), goats (Capra hircus, AB008754), sheep (Ovis aries, M73983), macaques (Macaca mulatta, EF208826), humans (Homo sapiens, NM_019111), pigs (Sus scrofa, M93028) and mice (Mus musculus, NM_010381). A hyphen (-) indicates amino acid identity and an asterisk (*) indicates a gap inserted for maximum alignment. Arrows (↓) within the green column indicate the amino acid positions constituting part of peptide-binding site (PBS). A circle (О) with a blue column indicates a conserved site for T-cell receptor interaction and putative N-linked glycosylation sites are underlined. A rectangle sign (

) denotes the position of residues associated with binding of CD4 molecules. S-S indicates a disulphide bond between two cysteine residues. Proposed DRA locus specific motifs within connecting peptide (CP), transmembrane (TM) and cytoplasmatic (CY) domains are indicated by pink, green and yellow areas, respectively.

Figure 2. Alignment of the protein encoded by the DRA genes in gayal (Bos frontalis), gaytle (B. frontalis × B. taurus), European cattle (B. taurus, D37956), zebu cattle (Bos indicus, FM986338), yak (Bos grunniens, JQ911700), Chinese yakow (B. grunniens × B. taurus, JQ347519), buffaloes (Bubalus bubalis, DQ016629), goats (Capra hircus, AB008754), sheep (Ovis aries, M73983), macaques (Macaca mulatta, EF208826), humans (Homo sapiens, NM_019111), pigs (Sus scrofa, M93028) and mice (Mus musculus, NM_010381). A hyphen (-) indicates amino acid identity and an asterisk (*) indicates a gap inserted for maximum alignment. Arrows (↓) within the green column indicate the amino acid positions constituting part of peptide-binding site (PBS). A circle (О) with a blue column indicates a conserved site for T-cell receptor interaction and putative N-linked glycosylation sites are underlined. A rectangle sign (Display full size) denotes the position of residues associated with binding of CD4 molecules. S-S indicates a disulphide bond between two cysteine residues. Proposed DRA locus specific motifs within connecting peptide (CP), transmembrane (TM) and cytoplasmatic (CY) domains are indicated by pink, green and yellow areas, respectively.

Figure 3. Phylogenetic tree for the DRA genes from gayal, gaytle, European cattle, zebu, yak, Chinese yakow, buffaloes, goats, sheep, macaques, humans, pigs, cats, dogs and mice.

Figure 3. Phylogenetic tree for the DRA genes from gayal, gaytle, European cattle, zebu, yak, Chinese yakow, buffaloes, goats, sheep, macaques, humans, pigs, cats, dogs and mice.