Figures & data
Figure 1. Content of phenolic acids from chloroform (A) and methanol (B) extracts and flavonoids (C) from methanol extracts of in vivo and in vitro propagated plants of Lamium album L. LaMeS in vivo – methanol extracts from in vivo L. album; LaChlS in vivo – chloroform extracts from in vivo L. album; LaMeS in vitro – methanol extracts from in vitro L. album; LaChlS in vitro – chloroform extracts from in vitro propagated L. album.
Figure 2. Morphological changes in A549 and MDCKII cells after 24-h treatment with methanol (Me) and chloroform (Chl) extracts from in vivo and in vitro propagated Lamium album L. plants.
Table 1. Effect of treatment with chloroform extracts on adhesion properties of cells.
Figure 3. Membrane permeability test with trypan blue exclusion. MDCKII cells (A, B and C) and A549 cells (D, E and F) after 24 h (A and D) and 48 h (B, C, E and F) treatment. 1 – control; 2 – control with DMSO; 3 – cells with methanol extracts from in vivo plants; 4 – cells with chloroform extracts from in vivo plants; 5 – cells with methanol extracts from in vitro plants; 6 – cells with chloroform extracts from in vitro plants.
Figure 4. Determination of TER of MDCK II (A) and A549 (B) cells. The figure shows arrest of TER of cells after administration of different Lamium album L. extracts in concentration 1 mg/ml. Met – methanol extracts; Chl – chloroform extracts; DMSO – control with DMSO; free – control without DMSO.
Figure 5. Changes in actin cytoskeleton of A549 (A) and MDCK II (B) cells. Fluorescence staining with phalloidin–TRITC, fluorescent microscopy, original magnification 600× (A) and 400× (B). control-DMEM – control without DMSO; control-DMEM+DMSO – control cells with DMSO; LaMeS in vivo – methanol extracts from in vivo L. album; LaChlS in vivo – chloroform extracts from in vivo L. album; LaMeS in vitro – methanol extracts from in vitro L. album; LaChlS in vitro – chloroform extracts from in vitro L. album.
