Optimization of carbon source and glucose feeding strategy for improvement of L-isoleucine production by Escherichia coli

Figures & data

Figure 1. Effects of carbon source on L-isoleucine fed-batch fermentation by E. coli TRFP in baffled flasks.

Figure 2. Effect of different initial sucrose concentrations on L-isoleucine fed-batch fermentation by E. coli TRFP in baffled flasks.

Figure 3. Effect of different feeding carbon sources on L-isoleucine fed-batch fermentation by E. coli TRFP.

Note: DCW (squares), production (circles) of L-isoleucine, concentration of acetate (upright triangles).

Figure 4. Effect of different DO concentrations on L-isoleucine fed-batch fermentation by E. coli TRFP with DO-stat feeding. Biomass (a); L-isoleucine (filled symbols) and acetate (open symbols) production (b).

Figure 5. L-isoleucine fed-batch fermentation by E. coli TRFP with glucose-stat feeding.

Figure 6. L-isoleucine fed-batch fermentation by E. coli TRFP with a combined feeding strategy of glucose-stat feeding and DO feedback feeding. Glucose-stat feeding (I); DO feedback feeding (II).