Molecular cloning, sequence characterization and expression pattern of Rab18 gene from watermelon (Citrullus lanatus)

Figures & data

Table 1. qRT-PCR primers for watermelon Rab18, actin genes and annealing temperature.

Gene	Primer sequence	Ta (°C)	Length (bp)
Rab18	Forward: 5′-TCACTTCCGATTCCTTTG-3′Reverse: 5′-ACCCGTTCACTTTCCTTA-3′	50	310
Actin	Forward: :5′-AAGGTCCAAACGGAGAAT-3′Reverse: 5′-CAACCCAAAGGCTAACAG-3′	51	211

Figure 1. RACE results for watermelon Rab18 gene. M, DL2000 DNA markers; 1, 3′-RACE product for watermelon Rab18 gene; 2, 5′-RACE product for watermelon Rab18 gene.

Figure 2. The complete mRNA of watermelon Rab18 gene and its encoding amino acids. * indicates the stop codon.

Figure 3. The alignment of the proteins encoded by watermelon and other Rab18 genes.

Figure 4. The putative Rab18 domain of the protein encoded by watermelon Rab18 gene.

Figure 5. The phylogenetic tree for seven kinds of Rab18 genes.

Figure 6. Expression analysis of Rab18 gene mRNA in various watermelon tissues: 1, leaves; 2, stem; 3, flower; 4, root; 5, fruit (immature white stage); 6, fruit (white-pink flesh stage); 7, fruit (red flesh stage); 8, fruit (over-ripe stage).