Plasmid DNA pre-purification by tangential flow filtration

Figures & data

Figure 1. Mode of operation diagram for the ultrafiltration system. Batch concentration (a); diafiltration (b); and total recirculation mode (c).

Figure 2. J variation as a function of ∆TMP. Plasmid concentrations: (■) 1.04 µg/mL, (▴) 3.81 µg/mL and (•) 4.69 µg/mL.

Table 1. Parameters for the ultrafiltration model.

	C1	C2	C3
Cb (μg/mL)	1.04	3.81	4.69
Jm (cm/s)	0.0042	0.0029	0.0023
∆TMP^++ (kg/cm^2)	0.34	0.31	0.23
R^2	0.9592	0.9891	0.9746

Figure 3. J variation as a function of ln(C_b). (•) 0.50 kg/cm², (■) 0.75 kg/cm², (♦)1.00 kg/cm², (▴) 1.25 kg/cm² and (×) 1.50 kg/cm².

Figure 4. Electrophoresis gel. Lane 1: molecular weight marker; Lane 2: feed solution 2X; Lane 3: permeate solution; and Lane 4: retentate solution.

Figure 5. Analytical chromatogram. F: feed solution; P: permeate solution; R: retentate solution.

Table 2. Peak integration area for the concentration operation.

Solution	pDNA	Impurities	Percentage removal
Feed	0.40	18.87	–
Retentate	0.40	11.15	–
Permeate	N/D	7.72	41