Construction of a molecular genetic map for hawthorn based on SRAP markers

Figures & data

Figure 1. The result of some polymorphism SRAP primers in parents.

Table 1. SRAP primers used in this study.

Forward primers	Reverse primers
me1 5′-TGAGTCCAAACCGGAGC-3′	em1 5′-GACTGCGTACGAATTAAT-3′
me2 5′-TGAGTCCAAACCGGATA-3′	em2 5′-GACTGCGTACGAATTAGC-3′
me3 5′-TGAGTCCAAACCGGAAG-3′	em3 5′-GACTGCGTACGAATTGCA-3′
me4 5′-TGAGTCCAAACCGGACC-3′	em4 5′-GACTGCGTACGAATTACG-3′
me5 5′-TGAGTCCAAACCGGAAT-3′	em5 5′-GACTGCGTACGAATTAAC-3′
me6 5′-TGAGTCCAAACCGGTGT-3′	em6 5′-GACTGCGTACGAATTGAC-3′
me7 5′-TGAGTCCAAACCGGTTG-3′	em7 5′-GACTGCGTACGAATTATG-3′
me8 5′-TGAGTCCAAACCGGTAG-3′	em8 5′-GACTGCGTACGAATTTGC-3′
me9 5′-TGAGTCCAAACCGGTCA-3′	em9 5′-GACTGCGTACGAATTTGA-3′
me10 5′-TGAGTCCAAACCGGAGG-3′	em10 5′-GACTGCGTACGAATTTAG-3′
me11 5′-TGAGTCCAAACCGGAGA-3′	em11 5′-GACTGCGTACGAATTTCG-3′
me12 5′-TGAGTCCAAACCGGAAA-3′	em12 5′-GACTGCGTACGAATTGTC-3′
me13 5′-TGAGTCCAAACCGGAAC-3′	em13 5′-GACTGCGTACGAATTGGT-3′
me14 5′-TGAGTCCAAACCGGGTA-3′	em14 5′-GACTGCGTACGAATTCAG-3′
me15 5′-TGAGTCCAAACCGGACG-3′	em15 5′-GACTGCGTACGAATTCTG-3′
me16 5′-TGAGTCCAAACCGGGGT-3′	em16 5′-GACTGCGTACGAATTCGG-3′
me17 5′-TGAGTCCAAACCGGCAT-3′	em17 5′-GACTGCGTACGAATTCCA-3′
me18 5′-TGAGTCCAAACCGGGAC-3′	em18 5′-GACTGCGTACGAATTGAT-3′
me19 5′-TGAGTCCAAACCGGACA-3′	em19 5′-GACTGCGTACGAATTCTC-3′
me20 5′-TGAGTCCAAACCGGACT-3′	em20 5′-GACTGCGTACGAATTCAT-3′
me21 5′-TGAGTCCAAACCGGCAG-3′	em21 5′-GACTGCGTACGAATTCTA-3′
me22 5′-TGAGTCCAAACCGGCTA-3′	em22 5′-GACTGCGTACGAATTCAA-3′
	em23 5′-GACTGCGTACGAATTCAC-3′
	em24 5′-GACTGCGTACGAATTCTT-3′

Table 2. The combination message of the primers used in this study.

Primer combination	Primer combination	Primer combination	Primer combination
em5me5	em18me20	em8me17	em3me16
em1me8	em19me20	em13me17	em7me16
em2me8	em15me20	em11me17	em9me16
em4m422	em10me21	em16me17	em14me16
em5me22	em5me21	em15me17	em17me16
em6me22	em6me21	em19me17	em5me19
em7me19	em3me21	em17me17	em10me17
em12me19	em2me21	em21me17	em22me20
em13me19	em14me21	em23me17	em6me19
em15me19	em19me19	em23me18	em8me18
em17me19	em3me18	em20me18	em21me20
em20me20	em5me18	em18me18

The ‘e’ indicates ‘em’ and the ‘m’ indicates ‘me’.

Figure. 2. Amplification results of primer e4+m22.

Figure 3. The genetic linkage map of ‘Damianqiu’.

Figure 4. The genetic linkage map of ‘Qiujinxing’.