Hepatoprotective effects of dual-coated and uncoated mixture of probiotics in rats

Figures & data

Figure 1. Change in body weight in normal and CCl₄-treated rats. Group I (CTL): normal control; Group II (PBS): CCl₄ control; Group III (silymarin): silymarin standard and CCl₄; Group IV (coated-PRO): dual-coated probiotics and CCl₄; Group V (uncoated-PRO): uncoated probiotics and CCl₄. Note: Values represent means from six determinations ± SD.

Table 1. Effect of dual-coated and uncoated probiotics on the gain of body weight and the organ weights in CCl₄ treated rats.

Parameters	Group I	Group II	Group III	Group IV	Group V
Body weight gain (g/d)	8.13 ± 0.44	6.77 ± 0.35^#	7.37 ± 0.32^#	7.8 ± 0.17*	7.3 ± 0.52
Relative liver weight (g liver/g BW)	49.90 ± 1.23	54.89 ± 0.70^#*	54.76 ± 0.28^#	52.15 ± 0.93^#*	53.55 ± 2.21^#*
Relative kidney weight (g kidney/g BW)	9.38 ± 0.98	10.69 ± 0.06	9.87 ± 0.54*	9.59 ± 0.87*	9.19 ± 0.79*
Relative spleen weight (g spleen/g BW)	4.83 ± 0.28	3.80 ± 0.52^#	4.09 ± 0.18^#	3.49 ± 0.40^#	4.20 ± 0.18^#

Note: Values are mean of six determinations ± SD. Group I: normal control; Group II: CCl₄ control, Group III: silymarin standard and CCl₄, Group IV: dual-coated probiotics and CCl₄, Group V: uncoated probiotics and CCl₄.

^# p < 0.05 vs. Group I, *p < 0.05 vs. Group II.

Figure 2. Effects of dual-coated and uncoated probiotics on SGOT (A) and SGPT (B) levels against CCl₄-induced hepatotoxicity in rats. Group I: normal control; Group II: CCl₄ control, Group III: silymarin standard and CCl₄; Group IV: dual-coated probiotics and CCl₄; Group V: uncoated probiotics and CCl₄. Note: Values represent means from six determinations ± SD. ^a–cMeans with different superscripts differ (p < 0.05) by ANOVA.

Figure 3. Histopathological changes in the liver tissue of normal and CCl₄-treated rats. (A) Group I: normal control rats; centrally located and round nuclei and homogeneous cytoplasm (arrows). (B) Group II: CCl₄-treated rats, cell necrosis (arrows). (C) Group III: rats treated with silymarin plus CCl₄. (D) Group IV: rats treated with dual-coated probiotics plus CCl₄. (E) Group V: rats treated with uncoated probiotics plus CCl₄. Note: H&E staining; magnification ×400; bar = 200 μm.