Identification, molecular characterization and expression analysis of RPL24 genes in three Cucurbitaceae family members: cucumber, melon and watermelon

Figures & data

Figure 1. Nucleotide and deduced amino acid sequences of the CmRPL24-01 gene.

Table 1. Catalogue of seven RPL24 genes in cucumber, melon and watermelon.

ID	Phytozome identifier	Chromosome	Start position (bp)	End position (bp)	Protein length (aa)	pI	Molecular weight (kDa)	Intron number	Homology with	Percentage homology/e-value
C. sativus (cucumber)
CsRPL24-01	Cucsa.168290	2	10,413,357	10,416,506	180	10.24	19.85	5	CmRPL24-01	97%/1e-94
CsRPL24-02	Cucsa.074330	3	4,176,063	4,178,366	191	9.74	21.38	3	CmRPL24-02	96%/3e-139
CsRPL24-03	Cucsa.223850	Scaffold01798	16	461	105	10.16	11.27	3
C. melo (Melon)
CmRPL24-01	MELO3C014296P1	Scaffold00022	1,285,495	1,291,664	159	10.04	17.62	4	ClaRPL24-02	93%/1e-110
CmRPL24-02	MELO3C016583P1	Scaffold00028	3,483,564	3,486,101	191	9.84	21.42	3	ClaRPL24-01	94%/7e-136
C. lanatus (watermelon)
ClaRPL24-01	Cla000465	1	2,989,062	2,991,546	191	9.86	21.5	3	CsRPL24-02	93%/7e-135
ClaRPL24-02	Cla006043	7	2,613,047	2,616,007	159	10.17	17.42	4	CsRPL24-01	89%/4e-89

Note: Number of amino acids (aa).

Figure 2. Exon/intron structures of the Cucurbitaceae RPL24 genes.

Figure 3. Phylogenetic tree of Cucurbitaceae RPL24 proteins.

Figure 4. Schematical representation of conserved motifs (obtained using MEME) in RPL24 proteins.

Table 2. Amino acid composition of the Cucurbitaceae RPL24 protein motifs.

Motif No.	Sites	e-value	Amino acid sequence composition of motif	Width (aa)	Domain
Motif 1	7	2.5e-101	[HV][IK][KS][QR][EG][EQ][GE][EH][EQ]G[GQ]I[FI][KT][IV]EAP[IL]H[SV]SNV[MQ] [LV][YI][DS][KP][EV][QT][GN][KV][PA][CS][RK]VG[HIY][KR]YLEDG[KS][KR]VR[VY]	50	KOW motif
Motif 2	3	9.6e-056	QSSMTALSLSSNSFLGQRLPPP[TI]IYAAP[IV]TSKEKPCLIVM[KR]LKRWERKEC	50	NA
Motif 3	6	5.5e-064	[HK][MW][HK][VI][KL][RV]GD[NT]V[KM][IV]I[AR]G[KR]DKG[EK][IT]G[EV]I[KT] [KR][IV][FI][KR][HT][NQ] [NS][KR]V[IV]V[EN][EG][IK]NL	41	KOW motif

Note: The single letters of the motif match that letter; the letter groups in square brackets match any letters in the group.

Figure 5. Predicated 3D structures of RPL24 proteins.

Figure 6. Expression profiles of CmRPL24-01 gene under drought stress in melon leaf tissue obtained with qPCR.