In vitro evaluation of antioxidant activity of some plant methanol extracts

Figures & data

Table 1. List of plants used in the study.

Botanical name	Family	Code	Specimen number (ISTE)	Plant part used	Districts of collection
Crataegus microphylla C. Koch	Rosaceae	E1	76223	Leaves	Gölcük-Bolu
Teucrium sandrasicum O. Schwarz (endemic)	Lamiaceae	E2	87526	Aerial parts	Köyceğiz-Muğla
Centaurea nerimaniae Ş. Kültür (endemic)	Asteraceae	E3	98163	Aerial parts	Mersin
Olea europaea L.	Oleaceae	E4	106286	Leaves	Şarköy-Tekirdağ
Salvia hypargeia Fisch.& Mey. (endemic)	Lamiaceae	E5	98205	Aerial parts	Mersin
Cotinus coggygria Scop.	Anacardiaceae	E6	80926	Leaves	Kırklareli
Hypericum kotschyanum Boiss. (endemic)	Hypericaceae	E7	98173	Aerial parts	Mersin
Nepeta italica L.	Lamiaceae	E8	98192	Aerial parts	Mersin
Stachys cretica L. subsp. vacillans Rech. fil	Lamiaceae	E9	98166	Aerial parts	Mersin
Scorzonera tomentosa L. (endemic)	Asteraceae	E10	98954	Aerial parts	Malatya
Origanum sipyleum L. (endemic)	Lamiaceae	E11	86060	Aerial parts	Mersin
Rosa damascena Miller	Rosaceae	E12	106285	Flowers	Isparta
Colchicum sanguicolle K.M. Perss (endemic)	Colchicaceae	E13	48868	Cormus	Antalya
Centaurea antiochia Boiss. var. praealta (Boiss. & Bal) Wagenitz (endemic)	Asteraceae	E14	98247	Aerial parts	Mersin

Table 2. Plants and their chemical constituents with known antioxidant activity.

Plants	Chemical constituents	References
Crataegus microphylla C. Koch	Flavonoids	Melikoğlu et al. [10]
Centaurea nerimaniae Ş. Kültür (endemic)	Phytochemical research has not been done yet
Salvia hypargeia Fisch.& Mey. (endemic)	Diterpenes	Topcu et al. [11]
Cotinus coggygria Scop.	Monoterpenes, hydrolysable tannins, gallic acid, methyl gallate, pentagalloyl glucose, flavonoids, aurones, glycosides of myricetin, quercetin and kaempherol	Marčetić et al. [12]
Scorzonera tomentosa L. (endemic)	Stigmasterol 3β-glucoside, β-sitosterol, lupeol, lupeol acetate, α-amyrin, hydrangenol, hydramacrophyllol	Sarı et al. [13]
Origanum sipyleum L. (endemic)	Apigenin, naringenin, rosmarinic acid, rutin, vitexin, carvacrol (phenolic monoterpene)	Ozkan et al. [14]
Rosa damascena Miller	Monoterpene alcohols, hydrocarbons	Basim and Basim [15]
Colchicum sanguicolle K.M. Perss (endemic)	Phytochemical research has not been done yet

Table 3. DPPH radical-scavenging activity of the methanolic extracts.

Extracts	Concentration* (µg/mL)	Absorbance (517 nm)	DPPH radicals scavenged (%)
E1	500	0.111 ± 0.0015	92.82 ± 0.79
E2	50	0.783 ± 0.001	15.53 ± 4.2
E3	500	0.718 ± 0.008	23.81 ± 2.38
E4	500	0.323 ± 0.018	71.84 ± 9.02
E5	500	0.306 ± 0.015	86.64 ± 9.27
E6	500	0.082 ± 0.002	95.69 ± 0.17
E7	50	0.809 ± 0.003	4.52 ± 0.47
E8	250	0.756 ± 0.005	13.5 ± 1.90
E9	50	0.792 ± 0.024	13.77 ± 4.28
E10	50	0.756 ± 0.016	10.8 ± 0.01
E11	500	0.139 ± 0.083	93.19 ± 3.80
E12	500	0.085 ± 0.002	95.67 ± 0.68
E13	250	0.779 ± 0.015	15.5 ± 3.40
E14	500	0.693 ± 0.007	26.7 ± 3.88
BHA	100	0.122 ± 0.0075	89.49 ± 4.63
Vitamin E	100	0.223 ± 0.011	72.01 ± 5.85
Ascorbic acid	100	0.075 ± 0.001	96.78 ± 0.24

*In vitro noncytotoxic concentration for the methanolic extracts against the Vero cell line [27].

Figure 1. Antioxidant activity of five methanolic plant extracts as measured by the FTC method at 500 nm and compared to standards (vitamin E and BHA).

Figure 2. Antioxidant activity of five methanolic plant extracts as measured by the TBA method at 532 nm and compared to standards (vitamin E and BHA).

Figure 3. Comparison between the antioxidant activity of the five extracts as evaluated by the FTC and TBA methods.

Melikoğlu G, Bitiş L, Meriçli AH. Flavonoids of Crataegus microphylla. Nat Prod Res. 2004;18(3):211–213.

 PubMed Web of Science ®Google Scholar

Topcu G, Turkmen Z, Schilling JK, et al. Cytotoxic activity of some anatolian Salvia extracts and isolated abietane diterpenoids. J Pharm Biol. 2008;46(3):180–184.

 Web of Science ®Google Scholar

Marčetić M, Božić D, Milenković M, et al. Antimicrobial, antioxidant and anti-inflammatory activity of young shoots of the smoke tree, Cotinus coggygria Scop. PTR. 2013;27(11):1658–1663.

 Web of Science ®Google Scholar

Sarı A, Zidorn C, Ellmerer EP, et al. Phenolic compounds from Scorzonera tomentosa L. HCA. 2007;90(2):311–317.

 Web of Science ®Google Scholar

Ozkan G, Sagdic O, Ekici L, et al. Phenolic compounds of Origanum sipyleum L. extract, and its antioxidant and antibacterial activities. J Food Lipids. 2007;14(2):157–169.

 Web of Science ®Google Scholar

Basim E, Basim H. Antibacterial activity of Rosa damascena essential oil. Fitoterapia. 2003;74(4):394–396.

 PubMed Web of Science ®Google Scholar

Artun FT, Karagoz A, Ozcan G, et al. In vitro anticancer and cytotoxic activities of some plant extracts on cancer and normal cell lines. Mitt-Klosterneuburg. 2015;65(4):55–64.

 Google Scholar