1,851
Views
3
CrossRef citations to date
0
Altmetric
Articles; Pharmaceutical Biotechnology

Production of diosgenin from Dioscorea zingiberensis with mixed culture in a new tray bioreactor

, , , &
Pages 158-164 | Received 05 May 2015, Accepted 11 Aug 2015, Published online: 07 Oct 2015

Figures & data

Figure 1. Two-dimensional (a) and three-dimensional (b) diagram of the new solid-fermentation bioreactor. 1: ultraviolet (UV) light source; 2: wet conditioner; 3: air conditioner; 4: aluminum shelves; 5: air pump; 6: perforated pipes; 7: tray; 8: sterilized filter; 9: water container; 10: gauze filter: 11: gas exit; and 12: rake.

Figure 1. Two-dimensional (a) and three-dimensional (b) diagram of the new solid-fermentation bioreactor. 1: ultraviolet (UV) light source; 2: wet conditioner; 3: air conditioner; 4: aluminum shelves; 5: air pump; 6: perforated pipes; 7: tray; 8: sterilized filter; 9: water container; 10: gauze filter: 11: gas exit; and 12: rake.

Figure 2. Kinetics of diosgenin production (a) in the new SSF reactor with different initial moisture content: 70% (left diagonal

), 75% (right diagonal
), 80% (horizontal stripes
) and 85% (black bars). Kinetics of medium moisture content loss (b) during SSF with different initial moisture content: 70% (
); 75% (
); 80% (
); and 85% (
). The standard deviations were less than 10%.

Figure 2. Kinetics of diosgenin production (a) in the new SSF reactor with different initial moisture content: 70% (left diagonal Display full size), 75% (right diagonal Display full size), 80% (horizontal stripes Display full size) and 85% (black bars). Kinetics of medium moisture content loss (b) during SSF with different initial moisture content: 70% (Display full size); 75% (Display full size); 80% (Display full size); and 85% (Display full size). The standard deviations were less than 10%.

Figure 3. Kinetics of diosgenin production (a) in the new SSF reactor at different incubation temperatures: 25 °C (left diagonal

), 30 °C (right diagonal
), 35 °C (horizontal stripes
) and 40 °C (black bars). Temperature changes (b) in the tray bed during SSF at different air-conditioning temperature: 25 °C (
); 30 °C (
); 35 °C (
); and 40 °C (
). The standard deviations were less than 10%.

Figure 3. Kinetics of diosgenin production (a) in the new SSF reactor at different incubation temperatures: 25 °C (left diagonal Display full size), 30 °C (right diagonal Display full size), 35 °C (horizontal stripes Display full size) and 40 °C (black bars). Temperature changes (b) in the tray bed during SSF at different air-conditioning temperature: 25 °C (Display full size); 30 °C (Display full size); 35 °C (Display full size); and 40 °C (Display full size). The standard deviations were less than 10%.

Figure 4. Kinetics of diosgenin production (a) in the new SSF reactor with different tray depths: 1 cm (left diagonal

), 1.5 cm (right diagonal
), 2 cm (horizontal stripes
) and 2.5 cm (black bars). Kinetics of medium moisture content loss (b) and temperature changes in tray bed (c) during SSF with different tray depths: 1 cm (
); 1.5 cm (
); 2 cm (
); and 2.5 cm (
). The standard deviations were less than 10%.

Figure 4. Kinetics of diosgenin production (a) in the new SSF reactor with different tray depths: 1 cm (left diagonal Display full size), 1.5 cm (right diagonal Display full size), 2 cm (horizontal stripes Display full size) and 2.5 cm (black bars). Kinetics of medium moisture content loss (b) and temperature changes in tray bed (c) during SSF with different tray depths: 1 cm (Display full size); 1.5 cm (Display full size); 2 cm (Display full size); and 2.5 cm (Display full size). The standard deviations were less than 10%.

Figure 5. Kinetics of diosgenin production (a) in the new SSF reactor with different mixing times: once (left diagonal

), two times (right diagonal
), three times (horizontal stripes
) and four times (black bars). Kinetics of medium moisture content loss (b) and temperature changes in tray bed (c) during SSF with different mixing times: once (
); two times (
); three times (
); and four times (
). The standard deviations were less than 10%.

Figure 5. Kinetics of diosgenin production (a) in the new SSF reactor with different mixing times: once (left diagonal Display full size), two times (right diagonal Display full size), three times (horizontal stripes Display full size) and four times (black bars). Kinetics of medium moisture content loss (b) and temperature changes in tray bed (c) during SSF with different mixing times: once (Display full size); two times (Display full size); three times (Display full size); and four times (Display full size). The standard deviations were less than 10%.

Figure 6. Kinetics of biomass growth (a) and enzyme activity (b) of α-rhamnase (

) and β-glucosidase (
) during mixed-culture SSF. Profiles (c) of concentrations of diosgenin-diglucoside (▴), diosgenin-glucoside-rhamnoside (
), diosgenin-glucoside (△) and diosgenin (☆) during mixed-culture SSF. The standard deviations were less than 10%.

Figure 6. Kinetics of biomass growth (a) and enzyme activity (b) of α-rhamnase (Display full size) and β-glucosidase (Display full size) during mixed-culture SSF. Profiles (c) of concentrations of diosgenin-diglucoside (▴), diosgenin-glucoside-rhamnoside (Display full size), diosgenin-glucoside (△) and diosgenin (☆) during mixed-culture SSF. The standard deviations were less than 10%.