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Biotechnology & Biotechnological Equipment Volume 30, 2016 - Issue 1
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Articles; Agriculture and Environmental Biotechnology

Heterologous expression and characterization of a laccase from Laccaria bicolor in Pichia pastoris

Bo WangShanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai201106, P.R. ChinaView further author information
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Xiuying WangShanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai201106, P.R. China;Department of Biological Sciences, College of Life and Environmental Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai200234, P.R. ChinaView further author information
,
Yongsheng TianShanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai201106, P.R. ChinaView further author information
,
Zhenjun LiShanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai201106, P.R. ChinaView further author information
,
Jianjie GaoShanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai201106, P.R. ChinaView further author information
,
Ying YanDepartment of Rice Breeding, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai201106, P.R. ChinaView further author information
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Rihe PengShanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai201106, P.R. ChinaView further author information
&
Quanhong YaoShanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai201106, P.R. ChinaCorrespondence[email protected]
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Pages 63-68 | Received 15 Jul 2015, Accepted 02 Oct 2015, Published online: 11 Nov 2015

Figures & data

Figure 1. SDS-PAGE of recombinant LBLCC3I expressed via P. pastoris.

Figure 1. SDS-PAGE of recombinant LBLCC3I expressed via P. pastoris.

Figure 2. Effects of pH on laccase activity (a) and stability (b).

Figure 2. Effects of pH on laccase activity (a) and stability (b).

Figure 3. Effects of temperature on laccase activity (a) and stability (b).

Figure 3. Effects of temperature on laccase activity (a) and stability (b).

Figure 4. Decolourization rate of malachite green, crystal violet and orange G.

Figure 4. Decolourization rate of malachite green, crystal violet and orange G.

Figure 5. Effects of metal ions (a) and inhibitors (b) on laccase activity.

Figure 5. Effects of metal ions (a) and inhibitors (b) on laccase activity.

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