Role of P2X₇ on steroid synthesis in murine luteal cells

Figures & data

Table 1. Oligonucleotides used for real-time PCR.

Gene	Primers
3β-HSD	5′CTCAGTTCTTAGGCTTCAGCAATTAC3′ (sense)
3β-HSD	5′GCCCAATTCCCAGACAGTAG3′ (antisense)
StAR	5′CCGGAGCAGAGTGGTGTCA3′ (sense)
StAR	5′CAGTGGATGAAGCACCATGC3′ (antisense)
CYP11A	5′CCAGTGTCCCCATGCTCAAC3′ (sense)
CYP11A	5′TGCATGGTCCTTCCAGGTCT3′ (antisense)
GAPDH	5′TCCTTGGAGGCCATGTAGGCCAT3′ (sense)
GAPDH	5′TGATGACATCAAGAAGGTGGTGAAG3′ (antisense)

Note: steroidogenic acute regulatory protein (StAR); P450 side-chain cleavage enzyme gene (CYP11A); 3β-hydroxysteroid dehydrogenase (3β-HSD); glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

Figure 1. Influence of P2X₇ purinergic signalling on (A) progesterone and (B) estradiol secretion in luteal cells.

Figure 2. Effect of BBG on progesterone secretion in luteal cells, induced by ATP and BzATP.

Figure 3. mRNA level of (A) CYP11A, 3β-HSD, StAR and (B) protein level of CYP11A in luteal cells assessed by real-time PCR and Western blotting.

Figure 4. The effect of BBG on CYP11A mRNA expression induced by ATP and BzATP.