Figures & data
Table 1. Alignment of the N-terminal sequence of NLCC1 with other thermotolerant laccases from different organisms.
Figure 1. 3D model comparison of native laccase NLCC1 (a) and mutant laccase MLCC1 (b).
Figure 2. Neighbouring amino acids in the mutation site on position 14 of NLCC1 (a) and MLCC1 (b).
Figure 3. Time course of the laccase activity (▴) and cell dry weight (■) through a high-density fermentation in 5 L fermenter.
Table 2. Purification of recombinant laccase.
Figure 4. SDS-PAGE of mutant laccase secreted by recombinant P. pastoris.
Figure 5. Effect of pH on the activity of mutant and native laccase (a) and pH tolerance of the mutant and the native laccase (b) with ABTS as substrate. Effect of temperature on the activity of mutant and native laccase (c) and temperature tolerance of the mutant and the native laccase (d) with ABTS as substrate.
Figure 6. Thermal stability of MLCC1 with ABTS as substrates.
Table 3. Optimal pH values for different MLCC1 substrates.
Table 4. Kinetic parameters of MLCC1 and NLCC1.