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Articles; Agriculture and Environmental Biotechnology

Cloning and expression analysis of eIF-5A gene in Apocynum venetum

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Pages 677-684 | Received 16 Sep 2015, Accepted 29 Mar 2016, Published online: 09 May 2016

Figures & data

Figure 1. Schematic representation of the polypeptide of AveIF-5A and AveIF-5A1.

Figure 1. Schematic representation of the polypeptide of AveIF-5A and AveIF-5A1.

Figure 2. Amino-acid sequence alignment (A) and phylogenetic analysis (B) of eIF-5A of Apocynum venetum and eIF-5A of other plants.

Figure 2. Amino-acid sequence alignment (A) and phylogenetic analysis (B) of eIF-5A of Apocynum venetum and eIF-5A of other plants.

Figure 3. Expression levels of the eIF-5A gene in leaves of Apocynum venetum under abiotic stress: low-temperature (5 °C) stress (A); salt (200 mmol/L NaHCO3) stress (B); and drought (20% PEG6000) stress (C).

Figure 3. Expression levels of the eIF-5A gene in leaves of Apocynum venetum under abiotic stress: low-temperature (5 °C) stress (A); salt (200 mmol/L NaHCO3) stress (B); and drought (20% PEG6000) stress (C).

Figure 4. Growth kinetics of recombinant E. coli cells expressing AveIF-5A and AveIF-5A1 grown in liquid nutrient medium in: normal conditions (A), low-temperature (16 °C) (B), 0.5 mol/L NaCl (C) and 20% PEG stress (D).

Figure 4. Growth kinetics of recombinant E. coli cells expressing AveIF-5A and AveIF-5A1 grown in liquid nutrient medium in: normal conditions (A), low-temperature (16 °C) (B), 0.5 mol/L NaCl (C) and 20% PEG stress (D).