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Biotechnology & Biotechnological Equipment Volume 30, 2016 - Issue 4
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Articles; Food Biotechnology

Construction of acetoin high-producing Bacillus subtilis strain

Yanjun TianState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, PR China;Food and Fermentation Engineering Key Lab of Shandong Province, Institute of Food and Fermentation, Shandong Food Fermentation Industry Research and Design Institute, Jinan, Shandong, PR China
,
Hui XuFood and Fermentation Engineering Key Lab of Shandong Province, Institute of Food and Fermentation, Shandong Food Fermentation Industry Research and Design Institute, Jinan, Shandong, PR China
,
Jianjun LiuFood and Fermentation Engineering Key Lab of Shandong Province, Institute of Food and Fermentation, Shandong Food Fermentation Industry Research and Design Institute, Jinan, Shandong, PR China
,
Wei ChenState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, PR China
,
Wentao SunFood and Fermentation Engineering Key Lab of Shandong Province, Institute of Food and Fermentation, Shandong Food Fermentation Industry Research and Design Institute, Jinan, Shandong, PR China
&
Yongquan ChenState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, PR ChinaCorrespondence[email protected]
Pages 700-705 | Received 15 Oct 2015, Accepted 13 Apr 2016, Published online: 09 May 2016

Figures & data

Table 1. Random primers used in this study.

Table 2. Rescreening results from flask fermentation.

Table 3. Stability of the mutant HB-32.

Table 4. Influence of preservation time at 4 °C in a refrigerator on the acetoin production by the mutant B. subtilis HB-32.

Figure 1. Electrophoretogram of PCR products amplified using different annealing temperatures.

Figure 1. Electrophoretogram of PCR products amplified using different annealing temperatures.

Figure 2. Amplification results of strain TH-49, AD-30 and HB-32.

Figure 2. Amplification results of strain TH-49, AD-30 and HB-32.

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