Analysis of genetic diversity of Sclerotinia sclerotiorum from eggplant by mycelial compatibility, random amplification of polymorphic DNA (RAPD) and simple sequence repeat (SSR) analyses

Figures & data

Table 1. Sclerotinia sclerotiorum isolates used in this study with indication of geographic origin (province and location), mycelial compatibility grouping (MCG), virulence (lesion length) and pattern of random amplified polymorphic DNA (RAPD) and haplotype of single strand polymorphism.

Isolate code	Location	Province	Lesion length (mm)	MCG	RAPD group	SSR group
Scl1	Ceyhan	Adana	56	1	1	1
Scl2	Ceyhan	Adana	32	1	2	2
Scl3	Ceyhan	Adana	37	1	1	2
Scl4	Ceyhan	Adana	83	1	1	3
Scl5	Center	Adana	66	2	1	1
Scl6	Center	Adana	82	2	1	3
Scl8	Center	Adana	40	2	1	2
Scl9	Center	Adana	90	2	2	3
Scl7	Center	Adana	38	3	2	2
Scl10	Kozan	Adana	62	4	2	1
Scl11	Kozan	Adana	100	4	1	3
Scl12	Kozan	Adana	32	4	1	2
Scl13	Kozan	Adana	91	5	2	3
Scl14	Demre	Antalya	65	6	2	1
Scl16	Demre	Antalya	93	6	1	3
Scl17	Demre	Antalya	63	6	2	1
Scl18	Demre	Antalya	102	6	1	3
Scl15	Demre	Antalya	32	7	1	2
Scl19	Kumluca	Antalya	30	8	2	2
Scl20	Kumluca	Antalya	90	8	1	3
Scl21	Kumluca	Antalya	60	9	1	1
Scl22	Kumluca	Antalya	23	10	2	2
Scl23	Center	Antalya	93	11	1	3
Scl24	Center	Antalya	31	11	2	2
Scl25	Center	Antalya	36	11	1	2
Scl26	Center	Antalya	68	11	1	1
Scl28	Manavgat	Antalya	94	11	1	3
Scl27	Manavgat	Antalya	37	12	2	2
Scl29	Manavgat	Antalya	36	12	1	2
Scl30	Manavgat	Antalya	30	12	2	2
Scl31	Kırıkhan	Hatay	85	13	1	3
Scl32	Center	Hatay	65	14	1	1
Scl33	Center	Hatay	85	14	2	3
Scl34	Center	Hatay	30	14	1	2
Scl35	Center	Hatay	30	14	1	2
Scl36	Center	Hatay	100	14	2	3
Scl37	Reyhanlı	Hatay	40	14	1	2
Scl38	Samandağ	Hatay	62	15	2	1
Scl39	Samandağ	Hatay	86	15	1	3
Scl40	Samandağ	Hatay	23	15	1	2
Scl41	Samandağ	Hatay	32	15	2	2
Scl42	Samandağ	Hatay	97	15	1	3
Scl43	Andırın	Kahramanmaraş	62	17	2	1
Scl44	Andırın	Kahramanmaraş	98	17	1	3
Scl45	Center	Kahramanmaraş	38	17	2	2
Scl46	Center	Kahramanmaraş	52	17	1	1
Scl47	Center	Kahramanmaraş	85	17	1	3
Scl48	Erdemli	Mersin	38	18	2	2
Scl49	Center	Mersin	34	19	1	2
Scl50	Center	Mersin	60	19	2	1
Scl51	Center	Mersin	28	19	1	2
Scl52	Silifke	Mersin	23	19	2	2
Scl53	Silifke	Mersin	39	20	1	2
Scl54	Tarsus	Mersin	85	20	2	3
Scl55	Tarsus	Mersin	70	21	1	1
Scl56	Center	Osmaniye	93	22	2	3
Scl57	Center	Osmaniye	52	22	1	1
Scl58	Center	Osmaniye	100	22	2	3
Scl59	Center	Osmaniye	20	22	1	2
Scl60	Center	Osmaniye	53	22	2	1
LSD (0.05)			3.4

Note: LSD, least significant difference.

Table 2. List of RAPD primers, observed band numbers and polymorphic band numbers.

Primer code	Primer sequence (5′–3′)	Band number	Polymorphic band number	Polymorphism (%)
OPA-01	CAGGCCCTTC	3	1	33
OPA-05	AGGGGTCTTG	4	2	50
OPA-18	AGGTGACCGT	5	1	20
OPB-01	GTTTCGCTCC	3	0	0
OPB-06	TGCTCTGCCC	3	1	33
OPB-14	TCCGCTCTGG	2	1	50
OPB-19	ACCCCCGAAG	3	1	33
OPC-02	GTGAGGCGTC	4	2	50
OPC-08	TGGACCGGTG	3	1	33
OPC-15	GACGGATCAG	5	3	60

Table 3. List of SSR primers, observed band numbers and polymorphic band numbers.

Repeat motif	Locus	Primer sequence (5′–3′)	Size range (bp)	Band number	Polymorphic band number
(GA)14	7	TTTGCGTATTATGGTGGGC ATGGCGCAACTCTCAATAGG	160–172	3	2
(CA)9	12	CGATAATTTCCCCTCACTTGC GGAAGTCCTGATATCGTTGAGG	215–225	5	3
(TTA)9	17	TCATAGTGAGTGCATGATGCC CAGGGATGACTTTGGAATGG	345–390	3	1
(TACA)10	55	GTTTTCGGTTGTGTGCTGG GCTCGTTCAAGCTCAGCAAG	173–221	4	2
(CT)12	92	TCGCCTCAGAAGAATGTGC AGCGGGTTACAAGGAGATGG	374–378	5	2
(TATG)9	110	ATCCCTAACATCCCTAACGC GGAGAATTGAAGAATTGAATGC	362–378	3	2

Figure 1. Un-weighted pair group method with arithmetic mean (UPGMA) dendrograms showing the genetic relationships between the 60 isolates of Sclerotinia sclerotiorum based on RAPD markers.

Figure 2. Un-weighted pair group method with arithmetic mean (UPGMA) dendrograms showing the genetic relationships between the 60 isolates of Sclerotinia sclerotiorum based on SSR markers.