Effects of sub-MIC antibiotic concentrations on biofilm production of Salmonella Infantis

Figures & data

Table 1. MIC values of tested S. Infantis strains.

Strain code	GEN*	KAN*	NAL*	NEO*	SPE*	STR*	TET*
DMC 7	R (≥32)	R (≥64)	R (≥128)	R (≥32)	R (≥128)	R (≥32)	R (≥32)
DMC 12	R (≥32)	R (≥64)	R (≥128)	R (≥32)	R (≥128)	R (≥32)	R (≥32)
DMC 20	R (≥32)	R (≥64)	R (≥128)	R (≥32)	R (≥128)	R (≥32)	R (≥32)
DMC 23	R (≥32)	R (≥64)	R (≥128)	R (≥32)	R (≥128)	R (≥32)	R (≥32)
DMC 40	R (≥32)	R (≥64)	R (≥128)	R (≥32)	R (≥128)	R (≥32)	R (≥32)
DMC 41	R (≥32)	R (≥64)	R (≥128)	R (≥32)	R (≥128)	R (≥32)	R (≥32)
DMC 57	R (≥32)	R (≥64)	R (≥128)	R (≥32)	R (≥128)	R (≥32)	R (≥32)
DMC 58	R (≥32)	R (≥64)	R (≥128)	R (≥32)	R (≥128)	R (≥32)	R (≥32)
DMC 70	R (≥32)	R (≥64)	R (≥128)	R (≥32)	R (≥128)	R (≥32)	R (≥32)
DMC 71	R (≥32)	R (≥64)	R (≥128)	R (≥32)	R (≥128)	R (≥32)	R (≥32)
DMC 75	R (≥32)	R (≥64)	R (≥128)	R (≥32)	R (≥128)	R (≥32)	R (≥32)
DMC 76	R (≥32)	R (≥64)	R (≥128)	R (≥32)	R (≥128)	R (≥32)	R (≥32)
DMC 77	R (≥32)	R (≥64)	R (≥128)	R (≥32)	R (≥128)	R (≥32)	R (≥32)

*GEN, gentamycin; KAN, kanamycin; NAL, nalidixic acid; NEO, neomycin; SPE, spectinomycin; STR, streptomycin; TET, tetracycline; R, resistant

Table 2. MBEC values of tested S. Infantis strains.

Antibiotics	MIC values (µg/mL)	MBEC values (µg/mL)
Gentamycin	>32	>64 (2 × MIC)
Kanamycin	>64	>512 (8 × MIC)
Nalidixic acid	>128	>1024 (8 × MIC)
Neomycin	>32	>384 (12 × MIC)
Spectinomycin	>128	>1280 (10 × MIC)
Streptomycin	>32	>320 (10 × MIC)
Tetracycline	>32	>128 (4 × MİK)

Figure 1. Biofilm production levels of DMC coded 13 S. Infantis strains following the incubation at 20, 28 and 37 °C on polystyrene plates.

Figure 2. Pellicle production levels of S. Infantis strains at 20, 28 and 37 °C for eight days.

Figure 3. Effect of sub-MIC of seven tested antibiotics on biofilm formation of S. Infantis DMC 12.