Possible lack of association between E469K polymorphism of ICAM-1 and non-obstructive azoospermia in south-east Turkey

Figures & data

Table 1. Primer sequences, annealing temperature, restriction enzyme and allele sizes used for the ICAM-1 gene E469K polymorphism.

Primer sequences	Annealing temperature (°C)	Restriction enzyme	Allele size	NCBI SNP^∗
F:5′-GGAACCCATTGCCCGAGC-3′ R:5′-GGTGAGGATTGCATTAGGTC-3′	56	BstUI	A: 223 G:136 + 87	rs5498

Note:^∗www.ncbi.nlm.nih.gov/gene.

Figure 1. PCR-RFLP products of ICAM-1 gene E469K polymorphism obtained by 3% agarose gel electrophoresis.

Table 2. Genotype and allele frequencies of the ICAM-1 gene among patients with idiopathic azoospermia and fertile controls.

	Patients (n = 111)		Controls (n = 114)
Genotypes	n	%	n	%	OR	95% CI	P-value
AA	30	27.03	42	36.84	1
AG	59	53.15	51	44.74	1.62	0.88–2.95	0.11
GG	22	19.82	21	18.42	1.46	0.68–3.13	0.32
Allele
A	119	53.61	135	59.21	1
G	103	46.39	93	40.79	1.25	0.86–1.82	0.23

Note: Control and patient group consistent with Hardy–Weinberg equilibrium (HWE) X² = 1.45, P = 0.22 and HWE X² = 0.52, P = 0.469, respectively.

OR, odds ratio; CI, confidence interval.