Phylogenetic analysis of the bacterial community in a crystallizer pond, Pomorie salterns, Bulgaria

Figures & data

Table 1. Frequencies of OTUs within the bacteria domain derived from the 16S rRNA sequences from а crystallizer pond from Pomorie saltern, Bulgaria*.

OTU	Accession no.	No. of clones	Cluster affiliation	Results from BLAST analysis: closest match, sequence no., identity, isolation source
PB-3	LN865056	5	Firmicutes, Clostridia	Uncultured Halanaerobiaceae bacterium clone 2P90, EF106367.1, 93%, Guerrero Negro saltern, Baja CA Sur, Mexico
PB-4	LN865057	21	Firmicutes, Clostridia	Uncultured bacterium clone CAB311344321, JX883040.1, 94%, hypersaline Lake Tyrrell, Victoria, Australia
PB-20	LN865062	9	Firmicutes, Clostridia	Uncultured bacterium clone CAB311345841, JX883149.1, 99%, hypersaline Lake Tyrrell, Victoria, Australia
PB-27	LN865066	3	Firmicutes, Clostridia	Uncultured Firmicutes bacterium clone CSS170, JX240715.1 99%, Arabian Sea
PB-13	LN865060	4	Firmicutes, Clostridia	Alkaliphilus transvaalensis strain SAGM1, NR_024748.1 99%
PB-16	LN865061	7	Firmicutes, Clostridia	Uncultured bacterium clone SINH934, HM128363.1, 97%, Tibetian lake
PB-49	LN865073	1	Firmicutes, Clostridia	Alkaliphilus sp. FatMR1, KJ159210.1, 96%, Prony Hydrothermal Field, New Caledonia
PB-32	LN865069	3	Firmicutes, Bacilli	Planomicrobium okeanokoites strain 01-5, JX094171.1, 99%
PB-45	LN865072	3	Firmicutes, Bacilli	Bacillus sp. NB2006, KJ852652.1, 97%, lime working site
PB-2	LN865055	20	Bacteroidetes	Uncultured bacterium clone BB69, HQ425188.1, 96%, Aran-Bidgol salt lake, Iran
PB-72	LN865077	3	Bacteroidetes	Uncultured bacterium clone DSFBPENV12bac_4B2, KC465653.1, 98%, hypersaline pools, Salton Sea
PB-60	LN865075	1	Bacteroidetes, Cytophaga	Uncultured organism clone SBZP_6113, JN539265.1, 99%, Guerrero Negro solar saltern, Baja CA Sur, Mexico
PB-12	LN865059	6	Proteobacteria, Alfaproteobacteria	Uncultured organism clone SBXZ_3635, JN432945.1, 98%, Guerrero Negro solar saltern, Baja CA Sur, Mexico
PB-22	LN865064	3	Proteobacteria, Alfaproteobacteria	Roseovarius sp. strain RP14, HE576991.1, 94%, solar saltern, Alicante, Spain
PB-38	LN865070	5	Proteobacteria, Alfaproteobacteria	Uncultured organism clone MAT-CR-P5-G03, EU246244.1, 98%, hypersaline microbial mat, Candeleria lagoon, Puerto Rico
PB-43	LN865071	1	Proteobacteria, Alfaproteobacteria	Uncultured bacterium clone E6aH02, DQ103614.1, 93%, saltern pond, Eilat, Israel
PB-9	LN865058	1	Proteobacteria, Gammaproteobacteria	Spiribacter sp. UAH-SP71, CP005990.1, 97%, saltern ponds, Santa Pola, Spain
PB-21	LN865063	1	Proteobacteria, Gammaproteobacteria	Algiphilus aromaticivorans strain DG1253, NR_115808.1, 97%
PB-61	LN865076	5	Proteobacteria, Gammaproteobacteria	Uncultured bacterium clone C157, KJ817701.1, 97%, saline lake  Ebinur, China)
PB-82	LN865078	3	Proteobacteria, Gammaproteobacteria	Halovibrio denitrificans strain C4-2, EU868859.1, 93%
PB-86	LN865079	3	Proteobacteria, Deltaproteobacteria	Uncultured bacterium clone P12_4H, KF814551.1, 96%, Guerrero Negro solar saltern, Mexico
PB-54	LN865074	2	Deinococcus–Thermus, Deinococci	Uncultured bacterium clone Kasin-B2-F07, HE604687.1, 92%, Hypersaline Sediments of Lake Kasin, Southern Russia
PB-1	LN865054	6	Candidate division SR1	Uncultured organism clone SBYN_2369, JN462129.1, 83%, Guerrero Negro, Baja CA Sur, Mexico

Note: Novel sequences (more than 3% phylogenetic distance), their closest phylogenetic neighbours and similarity among them and their relative abundance are underlined.

Figure 1. Rarefaction curve for sampling of PS 16S rRNA bacterial gene library.

Figure 2. Genus affiliation of 16S rDNAs clones obtained from Pomorie salterns.

Table 2. Comparison of the microbial diversity in Pomorie saltern compared to other thalassohaline hypersaline ecosystems.

Saltern	Number of OTUs presented	Division presented (%)	Number of genera presented	Dominant genera^a(%)	New sequences^b (%)	Reference
Pomorie	26	Firmicutes Proteobacteria Bacteroidetes Deinococcus–Thermus Candidate division SR1	15	Halanaerobium, 33 Uncult. Bacteroidetes, 22	42.3	This study
Maras saltern	7	Gammaproteobacteria	3	Pseudomonas, 100	0	[20]
Guerrero Negro saltern (saltern ponds 11 and 12)	12	Proteobacteria Bacteroidetes	4	Psychroflexus, 16% (Pond 11)	n.d.	[21]
Tunisian multipond solar saltern	44	Bacteroidetes Proteobacteria	5	Salinibacter, 32	25	[22]
Santa Pola salterns (32% salt pond)	Bacteroides	11	5	Salinibacter, 72	n.d.	[18]

^aPhylogenetic groups representing more than 10% of the community sequences were determined as dominant.

^bSequences with less than 97% similarity to the closest neighbour were referred as new sequences.

Figure 3. Neighbour-joining phylogenetic tree based on bacterial 16S rDNA sequences found in a crystallizer pond, PS.

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