Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS

Figures & data

Figure 1. The test tubes filled with AH Plus and Obtuseal sealer.

Figure 2. The insertion of test tubes into the incision site with the aid of a presel.

Table 1. Work specification resume for ICP-MS Agilent 7500a and measurement parameters.

Parameter	Value	Units
RF power	1230	W
RF matching	1.7	V
Sample depth	7.6	mm
Torch	−1.2	mm
Torch-V	0.8	mm
Carrier gas	1.11	L/min
Make up gas	0.00	L/min
Nebulizer pump	0.11	Rps
Spray chamber temperature	2	deg ^oC
Ar flow rate	15	L/min
Auxiliary gas flow rate	0.9	L/min
Nebulizer gas flow rate	1.11	L/min
Sample uptake rate	0.3 rps	µL/min
Number of replicates	3
Integration time	3	s
Internal standards (^9Be,^45Sc,^103Rh,^208Bi)	200	ppb

Table 2. Concentrations (µg⋅kg⁻¹) of six elements in rat organs (n = 5).

		OB mean ± SE	AH Plus mean ± SE	Control mean ± SE	p
Brain	Mg	94192,0 ± 4752,6^a	81506,0 ± 1580,3^b	43150,0 ± 2677,8^c	0,009
	Al	56,2 ± 16,8	85,7 ± 16,0	76,8 ± 3,72	0,339
	Ca	1017,9 ± 444,9	1040,4 ± 79,0	1074,6 ± 123,1	0,212
	Cr	9,7 ± 0,8^a	10,8 ± 0,5^a	4,7 ± 0,3^b	0,030
	As	2,5 ± 0,6	2,3 ± 0,8	0,9 ± 0,4	0,239
	Pb	0,3 ± 0,09	0,4 ± 0,05	0,3 ± 0,1	0,875
Kidney	Mg	98458,0 ± 5364,4^a	97600,0 ± 3132,4^a	11485,3 ± 8425,4^b	0,039
	Al	31,9 ± 4,4	104,8 ± 34,9	61,0 ± 23,7	0,089
	Ca	442,6 ± 30,4	580,1 ± 60,0	386,7 ± 42,5	0,073
	Cr	12,7 ± 0,7^a	11,4 ± 0,6^a	5,7 ± 0,6^b	0,027
	As	7,1 ± 1,3^a	12,2 ± 2,3^a	3,2 ± 1,4^b	0,026
	Pb	0,8 ± 0,2	2,3 ± 0,8	2,1 ± 0,4	0,205
Liver	Mg	131680,0 ± 18131,8^a	126100,0 ± 3994,1^a	39516,6 ± 13639,5^b	0,031
	Al	89,0 ± 14,3	86,7 ± 21,5	274,1 ± 108,5	0,060
	Ca	343,4 ± 38,2	367,6 ± 25,2	322,5 ± 47,9	0,587
	Cr	10,8 ± 0,7^a	11,4 ± 0,2^a	4,8 ± 0,2^b	0,037
	As	15,4 ± 3,4^a	20,0 ± 2,5^a	3,6 ± 0,4^b	0,037
	Pb	0,4 ± 0,1^a	1,0 ± 0,1^b	0,9 ± 0,05^a	0,020

Note: Same letters in same lines demonstrate no statistically difference.

Bold values are statistically significant.