Methyl jasmonate induces triterpenoid biosynthesis in Inonotus baumii

Figures & data

Figure 1. Triterpenoid biosynthetic pathway of I. baumii.

Table 1. Primer sets used for quantitative real-time PCR.

Target gene	Primer sequence	Predicted product size (bp)
Ib-hmgs	Forward 5ˊ-CGAGAAGACTGCGAGGCGATAC-3ˊ Reverse 5ˊ-TGGGACTTGAGGGATTGGAGAGG-3ˊ	193
Ib-hmgr	Forward 5ˊ-GAAGAGGAGGAAGGCGTATGCGG-3ˊ Reverse 5ˊ-TGGAATGAAGCATGTTCGGAG-3ˊ	108
Ib-fpps	Forward 5ˊ-CCGTGGCATTTCCGTAACAGAC-3ˊ Reverse 5ˊ-CAGCAGGGTTGTCCTCTCCGTGT-3ˊ	174
Ib-sqs	Forward 5ˊ-TCCTGCTGCTCATCACACATCCTC-3ˊ Reverse 5ˊ-CCAATAAGTGCCAACATCGCTTCA-3ˊ	150
Ib-se	Forward 5ˊ-TAAGAACTCGGGGACTGAAACGAAG-3ˊ Reverse 5ˊ-CTTGAGAATCGCACCGACGAAGTAG-3ˊ	151
Ib-ls	Forward 5ˊ-ACACAGTTCGCCCTTGAGAGCC-3ˊ Reverse 5ˊ-CATCTTCACGGCCCGTTCGATAGGT-3ˊ	208
α-tubulin	Forward 5ˊ-CCAGCAAGCGTTACCGATT-3ˊ Reverse 5ˊ-TCCACGACGTCCATCGTTC-3ˊ	129
β-tubulin	Forward 5ˊ-GCTGAATATCGTTCGTGCCC-3ˊ Reverse 5ˊ-ATCCGCCTTCCTCCTTACAGT-3ˊ	170

Table 2. Triterpenoids content in I. baumii treated with different concentrations of MeJA for 48 h.

Treatment	Triterpenoids content (mg/g)^a	Significance^b
		a = 0.05	a = 0.01
Untreated control	3.11 ± 0.08	A	A
Control with ethanol	3.66 ± 0.04	B	b
50 μmol/L MeJA	3.05 ± 0.21	A	a
100 μmol/L MeJA	11.26 ± 0.28	C	c
150 μmol/L MeJA	12.61 ± 0.08	D	d
200 μmol/L MeJA	10.97 ± 0.11	C	c
250 μmol/L MeJA	6.39 ± 0.19	E	e

^aMean values with standard deviation (±SD). Triterpenoid contents were calculated as betulin equivalent from a standard curve using betulin.

^bThe multiple comparisons was determined by the LSD method. Different letters stand for significant variances. When a = 0.05, p < 0.05. When a = 0.01, p < 0.01.

Figure 2. Quantitative real-time PCR analysis of genes involved in the triterpenoid biosynthetic pathway.

Figure 3. Quantitative real-time PCR analysis of genes involved in the triterpenoid biosynthetic pathway.