The rs1043994 and rs3815188 genetic variations of the NOTCH3 gene and risk of type 2 diabetes mellitus

Figures & data

Table 1. PCR amplification primers and restriction endonucleases.

Single-nucleotide polymorphism	Base exchange	Oligonucleotide primer sequences	Enzyme
rs1043994	A684G	F: 5′-TAG TCG GGG GTG TGG TCA GT-3′ R: 5′-CCT CTG ACT CTC CTG AGT AG-3′	MwoI (60 °C for 30 min)
rs3815188	C381T	F: 5′-TGT GCT GCC CAA CCA AGC CA-3′ R: 5′-ACT GAC CAC ACC CCC GAC TA-3′	AciI (37 °C for 1 h)

Table 2. Genotype frequencies of the NOTCH3 rs1043994 variant.

		NOTCH3 rs1043994 genotypes
		AA		AG		GG
Groups	n	N	%	n	%	n	%
Control	100	43	43.0	53	53.0	4	4.0
T2DM	100	7	7.0	62	62.0	31	31.0
Total	200	50	25.0	115	57.5	35	17.5
Statistics		χ^2 = 47.453; p = 0.000

Table 3. Risk assessment for the NOTCH3 rs1043994 genotypes.

Genotypes	Odds ratio	95% CI	p
AA vs. AG	7.186	2.984–17.307	p < 0.05
AA vs. GG	47.607	12.815–176.865	p < 0.05
AG vs. GG	6.625	2.197–19.982	p < 0.05

95% CI, 95% confidence interval.

Table 4. Genotype frequencies of the NOTCH3 rs3815188 variant.

		NOTCH3 rs3815188 genotypes
		CC		CT		TT
Groups	n	N	%	n	%	n	%
Control	100	21	21.0	76	76.0	3	3.0
T2DM	100	69	69.0	22	22.0	9	9.0
Total	200	90	45.0	98	49.0	12	6.0
Statistics		χ^2 = 58.355; p = 0.000

Table 5. Risk assessment for the NOTCH3 rs3815188 genotypes.

Genotypes	Odds ratio	95% CI	p
CC vs. CT	11.351	5.745–22.427	p < 0.05
CC vs. TT	1.095	0.271–4.419	p > 0.05
TT vs. CT	10.364	2.581–41.614	p < 0.05