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Article; Biotechnological Equipment

Highly selective detection of Escherichia coli O157:H7 based on micro-gapped interdigitated electrode arrays

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Pages 1070-1078 | Received 24 Feb 2017, Accepted 23 May 2017, Published online: 30 May 2017

Figures & data

Figure 1. Schematic of procedures for preparing lithographic microelectrode chips.

Figure 1. Schematic of procedures for preparing lithographic microelectrode chips.

Figure 2. The design drawing of lithographic microelectrode chip.

Figure 2. The design drawing of lithographic microelectrode chip.

Figure 3. Front (A) and back (B) photographs of a 96-well combined microelectrode chip and a micrograph of a single microelectrode (C) under an optical microscope.

Figure 3. Front (A) and back (B) photographs of a 96-well combined microelectrode chip and a micrograph of a single microelectrode (C) under an optical microscope.

Figure 4. Schematic illustration of the microelectrode-based immunosensor for the detection of E. coli O157:H7.

Figure 4. Schematic illustration of the microelectrode-based immunosensor for the detection of E. coli O157:H7.

Figure 5. Clean (A), sediment appearance in gap (B) under ordinary optical microscope × 10. Clean (C), sediment appearance in gap (Dt) under scanning electron microscope.

Figure 5. Clean (A), sediment appearance in gap (B) under ordinary optical microscope × 10. Clean (C), sediment appearance in gap (Dt) under scanning electron microscope.

Figure 6. Sediment appearance in gap (A), scanning electron microscope. Electrode element display of Au (B) under energy-dispersive spectroscopy. Sediment appearance display of Ag (C) under energy-dispersive spectroscopy.

Figure 6. Sediment appearance in gap (A), scanning electron microscope. Electrode element display of Au (B) under energy-dispersive spectroscopy. Sediment appearance display of Ag (C) under energy-dispersive spectroscopy.

Figure 7. The LSV curves obtained by altering the concentrations of E. coli O157:H7 in PBST.

Figure 7. The LSV curves obtained by altering the concentrations of E. coli O157:H7 in PBST.

Figure 8. The selectivity of our assay obtained upon addition of various bacteria, including Hemolytic streptococcus, Staphylococcus aureus, Salmonella, Pseudomonas aeruginosa, Chronobacter sakazakii and Bacillus cereus at 108 CFU/mL.

Figure 8. The selectivity of our assay obtained upon addition of various bacteria, including Hemolytic streptococcus, Staphylococcus aureus, Salmonella, Pseudomonas aeruginosa, Chronobacter sakazakii and Bacillus cereus at 108 CFU/mL.