Screening and identification of probiotic Lactobacillus for the production of diosgenin from Dioscorea zingiberensis Wright by biotransformation

Figures & data

Table 1. PCR reaction conditions.

Reaction system	Reaction conditions
Supernatant (after denaturation)	1 μL	94 °C	5 min	1 cycle
PCR premix	25 μL	94 °C	1 min
Forward primer (20 pmol/μL)	0.5 μL	55 °C	1 min	30 cycles
Reverse primer2 (20 pmol/μL)	0.5 μL	72 °C	1.5min
16S-free H2O	23 μL	72 °C	5 min	1 cycle
Total volume	50 μL

Table 2. Sequencing primers.

Primer	Primer sequence
Seq forward	5′-GAGCGGATAACAATTTCACACAGG-3′
Seq reverse	5′-CGCCAGGGTTTTCCCAGTCACGAC-3′
Seq internal	5′-CAGCAGCCGCGGTAATAC-3′

Figure 1. Conversion rate of Lactobacillus strains at the end of the biotransformation process.

Figure 2. Fermentation broth pH values at the end of the biotransformation process.

Figure 3. Agarose gel electrophoresis of PCR products from genomic DNA isolated from representative probiotic strains.

Figure 4. Phylogenetic tree of concatenated Lactobacillus strain L20 (A), L35 (B), L83 (C) and L33 (D) constructed by the neighbour-joining method with Kimura 2-parameter as evolution model and based on 16S rDNA nucleotide sequences.