Gene and expression analysis of the hexamerin family proteins from the grasshopper, Locusta migratoria (Orthoptera: Acridoidea)

Figures & data

Table 1. Primers used in this study.

Primer name	Primer sequence (5’→3’)	Primer name	Primer sequence (5’→3’)
Hx1F	CTGGACACCGAACTCAAG	X-Hx1-5'outer	TCGGGCAGATGGTTGGAC
Hx1R	CGTCAGGCTCTTCACCTC	X-Hx1-5'inner	GTAGAAGGGCGGGATGAC
Hex2F	TGGTBTCVTACTTYACCG	X-Hx2-3'outer	CAACGCCCTGACCTTATC
Hex2R	GCGCTTGATBTCRTTCTC	X-Hx2-3'inner	GTCGTCCGAGTCTTCATTGG
Hx3F	GCCTTACACTTATGAGGATTTG	X-Hx2-5'outer	CGACAATGTGACCAAATAGG
Hx3R	TAGTTGATGGGAAAGTGGGT	X-Hx2-5'inner	CAGTTGTTGGAGGACGAAG
Hx4F	ACTCCCTCAGGCAGATGTT	X-Hx3-3'outer	CATCGTCACGCAGGCCTC
Hx4R	TCGTATCGTGGTCCATAGTAGA	X-Hx3-3'inner	GAGTTCAACGTGCCCAAC
Hx5-123F	GTCCAGAGGGTCTCAAGG	X-Hx3-5'outer	GAACTTGTCCTCGCCCTTG
Hx5-495R	TTCTTGTAGCCGTCCATTA	X-Hx3-5'inner	CTTCCTTCTCGCTCTTCAC
Hx-F1	GACCACTTCTACTACGGCATC	X-Hx4-3'outer	CAGAACTACCTTGAACCCTAC
Hx-R1	TAGGAGCAACTCGTCTCGT	X-Hx4-3'inner	CCACGACTCAACCACAAGC
XF-Hx-3'outer	GACCCACTTAGCATTGAG	X-Hx4-5'outer	GTAGGGTTCAAGGTAGTTCTG
XF-Hx-3'inner	CTGCTGAAGTACCTGGGAC	X-Hx4-5'inner	TGCAGGACGTGAGGACAT
XF-Hx-5'outer	CTGTCCAGTTGTGAGGGT	X-Hx5-3'outer	TGATTGGTCGCACGCTAC
XF-Hx-5'inner	CCTCCCAAAGCCTCCTCT	X-Hx5-3'inner	CACAATCTTCGGGCGTATG
X-Hx1-3'outer	GCTGTCCAACCATCTGC	X-Hx5-5'outer	ACGCCCGAAGATTGTGAAC
X-Hx1-3'inner	CTCACAACTGGACAGACC	X-Hx5-5'inner	GTACTGCAGAATCCAGGT

Table 2. Primers used in qRT-PCR.

Primer name	Sequences of primer (5'−3')	Primer name	Sequences of primer (5'−3')
β-actinF(1)	GCTCGTCCAAGGCATCAG	q-Hx3-F	TGCTGCTTCGCGAGGACAC
β-actinR(1)	TCCATGTCGTCCCAGTTTG	q-Hx3-R	CTCTCAGGTAGGCGTTGT
qRT-F-hex2	CAACGCCCTGACCTTATC	q-Hx4-F	GAGATGCTGCCCATAAACC
Q-XF-R2	CTCCTCCAGGCTGTATTCG	q-Hx4-R	CGTAGTCCTTGGCGTTGT
q-Hx1-F1	CTCAACCAGGGACTCTTCG	q-Hx5-F	CACAATCTTCGGGCGTAT
q-Hx1-R1	GATCACGTACGGCTTGCTCT	q-Hx5-R	CTCTTGTAGCCGTCCATT

Figure 1. Multiple alignment of amino acid sequences of the LmiHx protein in L.migratoria.

Figure 2. The putative conserved domain of the LmiHx protein in L. migratoria: LmiHx1(A); LmiHx2 (B); LmiHx4 (C); LmiHx5 (D).

Table 3. Physical and chemical parameters of the LmiHx protein in L. migratoria.

Name	Number of amino acids	Theoretical molecular weight (KD)	Theoretical pI value	Amount of negative charged amino acid residues (Asp + Glu)	Amount of positive charged amino acid residues (Arg + Lys)	Rate of aromatic amino acids (%)	Rate of methionine (%)
LmiHx1	657	75.95	6.28	87	79	15.30	0.20
LmiHx2	673	78.72	5.88	90	77	16.80	0.10
LmiHx4	685	79.03	5.97	81	74	12.90	3.90
LmiHx5	678	77.54	5.87	84	74	12.10	2.80

Figure 3. Predicted tertiary structure of the LmiHx protein in L. migratoria: LmiHx1(A); LmiHx2 (B); LmiHx4 (C); LmiHx5 (D).

Figure 4. Real-time qPCR analysis of the relative expression of LmiHx family genes at different developmental stages of L. migratoria.

Figure 5. Real-time qPCR analysis of the relative expression of LmiHx family genes in different tissues of L.migratoria adult males (A) and females (B).

Figure 6. Expression of LmiHx family genes after dsRNA injection. (A) LmiHx5, (B) LmiHx1; (C) LmiHx2; (D) LmiHx4.