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Article; Agriculture and Environmental Biotechnology

Genome-wide identification and analyses of the rice OsDUF936 family

, , , , , , , , & ORCID Icon show all
Pages 309-315 | Received 25 Mar 2017, Accepted 01 Dec 2017, Published online: 08 Dec 2017

Figures & data

Table 1 . OsDUF936 gene family and their predicted protein structure information.

Figure 1. Phylogenetic relationship of OsDUF936 members in rice and seven DUF936 members in Arabidopsis.

Figure 1. Phylogenetic relationship of OsDUF936 members in rice and seven DUF936 members in Arabidopsis.

Figure 2. Real-time PCR analysis of OsDUF936 gene family members in different tissues of rice Nipponbare. Fifteen representative tissues are as follows: Lb1, leaf blade at four-leaf stage; Lb2, leaf blade from plants with four tillers; Lb3, leaf blade at ripening stage; Ls1, leaf sheath at four-leaf stages; Ls2, leaf sheath from plants with four tillers; Rt1, root at four-leaf stages; Rt2, root at from plants with four tillers; St1, stem from plants with four tillers; St2, stem at ripening stage; An, 1.2–1.5 mm anther; Pi, pistil from 10 to 14 cm inflorescence; Em1, embryo at 7 days after flowering; Em2, embryo at 28 days after flowering; En1, endosperm at 7 days after flowering; En2, endosperm at 28 days after flowering.

Figure 2. Real-time PCR analysis of OsDUF936 gene family members in different tissues of rice Nipponbare. Fifteen representative tissues are as follows: Lb1, leaf blade at four-leaf stage; Lb2, leaf blade from plants with four tillers; Lb3, leaf blade at ripening stage; Ls1, leaf sheath at four-leaf stages; Ls2, leaf sheath from plants with four tillers; Rt1, root at four-leaf stages; Rt2, root at from plants with four tillers; St1, stem from plants with four tillers; St2, stem at ripening stage; An, 1.2–1.5 mm anther; Pi, pistil from 10 to 14 cm inflorescence; Em1, embryo at 7 days after flowering; Em2, embryo at 28 days after flowering; En1, endosperm at 7 days after flowering; En2, endosperm at 28 days after flowering.

Figure 3. Relative expression levels of OsDUF936 gene family members in rice Nipponbare seedlings under various stress and ABA conditions. D0, D1, D2 and D3: drought stress treatment for 0, 4, 8 and 16 h, respectively; S0, S1, S2 and S3: salt stress treatment for 0, 4, 8 and 16 h, respectively; C0, C1, C2 and C3: cold stress treatment for 0, 4, 8 and 16 h, respectively; H0, H1, H2 and H3: heat stress treatment for 0, 4, 8 and 16 h, respectively; A0, A1, A2 and A3: ABA treatment for 0, 4, 8 and 16 h, respectively.

Figure 3. Relative expression levels of OsDUF936 gene family members in rice Nipponbare seedlings under various stress and ABA conditions. D0, D1, D2 and D3: drought stress treatment for 0, 4, 8 and 16 h, respectively; S0, S1, S2 and S3: salt stress treatment for 0, 4, 8 and 16 h, respectively; C0, C1, C2 and C3: cold stress treatment for 0, 4, 8 and 16 h, respectively; H0, H1, H2 and H3: heat stress treatment for 0, 4, 8 and 16 h, respectively; A0, A1, A2 and A3: ABA treatment for 0, 4, 8 and 16 h, respectively.

Figure 4. Growth curve of E. coli recombinants overexpressing OsDUF936.6 under salt conditions.

Figure 4. Growth curve of E. coli recombinants overexpressing OsDUF936.6 under salt conditions.
Supplemental material

Supplemental_Data.pdf

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