Figures & data
Figure 1. Nucleotide and encoded amino acid sequences of BnGC4H in ramie.
Figure 2. Analysis of protein sequence of BnGC4H. (A) A conserved domain in BnGC4H of ramie. (B) Fifteen phosphorylation sites identified by NetPhos 2.0 prediction. (C) The sequence positions of predicted phosphorylation sites by NetPhos 2.0.
Figure 3. Phylogenetic analysis of BnGC4H among different plants. (A) Coding region amino acid sequence alignment of BnGC4H in ramie and homologous genes. The sequences of Nelumbo nucifera (accession no. XP_010253 046.1), Gossypium raimondii (accession no. XP_012454 096.1) and Gossypium hirsutum (accession no. NP_001314 057.1) were downloaded from the NCBI GenBank database. (B) Phylogenetic tree of C4H from ramie and other plant species. The sequences of Nicotiana tabacum (C4H1) ABC69412; Nicotiana tabacum (C4H2) ABC69411; Petunia hybrida (C4H1) ADX33332; Petunia hybrida (C4H2) ADX33333; Arabidopsis thaliana (C4H) AAC99993; Brassica napus (C4H1) DQ485129; Brassica napus (C4H2) DQ485131; Gossypium arboreum (C4H1) AAG10197; Gossypium arboreum (C4H2) AAG10196; Gossypium hirsutum (C4H1) FJ848866; Gossypium hirsutum (C4H2) FJ848867; Epimedium sagittatum (C4H) AIS92509.1; Ruta graveolens (C4H) AAN63028.1; Aquilaria sinensis (C4H) AGP25594.1; Boehmeria nivea (C4H) KY937946; Miscanthus giganteus (C4H1) ANB43563.1; Miscanthus giganteus (C4H2) ANB43564.1; Panicum virgatum (C4H1) AFY17064.1; Panicum virgatum (C4H2) AFY17065.1; Sorghum bicolor (C4H) AAK54447.1; Zea mays (C4H) NP001151365.1; Oryza sativa Japonica Group (C4H) BAF45113.1 were downloaded from the NCBI GenBank database.
Figure 4. Relative expression levels of BnGC4H during different developmental stages in the xylem and phloem of ramie. *P < 0.05.
Figure 5. In situ hybridization (ISH) of BnGC4H transcripts during different developmental stages of the ramie stem. Transverse sections hybridized with antisense probes at young stage (A and B), maturity stage (C and D) and late maturity stage (E and F).
