Production of nisin and lactic acid from corn stover through simultaneous saccharification and fermentation

Figures & data

Figure 1. Flow chart of the process used to assess SSF of pretreated corn stover to produce nisin and lactic acid in an integrated process.

Figure 2. Effect of cellulose on simultaneous saccharification and fermentation for nisin and lactic acid production.

Figure 3. Effect of enzyme (cellulase) addition on simultaneous saccharification and fermentation for nisin and lactic acid production from pretreated corn stover.

Figure 4. Effect of initial pH value on simultaneous saccharification and fermentation for nisin and lactic acid production from pretreated corn stover.

Table 1. Effect of peptone and yeast extract (YE) on the production of lactic acid and nisin.

	Nisin		Lactic acid
Nitrogen source and concentration (g/L)	Activity (IU/mL) ± SD^b	mg/L^a ± SD	g/L ± SD
10 peptone	1121 (±56.05)	28.04 (±1.40)	8.37 (±0.42)
10 YE	1241 (±74.46).	31.03 (±1.86)	8.68 (±0.35)
10 peptone + 10 YE	1468 (±58.72)	36.71 (±1.47)	10.14 (±0.51)
10 peptone +30 YE	1457 (±72.85).	36.44 (±1.82)	9.65 (±0.39)
30 peptone +10 YE	1437 (±57.48)	35.94 (±1.44)	9.42 (±0.47)
30 peptone +30 YE	1479 (±59.16)	36.98 (±1.48)	9.83 (±0.39)

^a Nisin concentration (mg/L) = (x) Ч 0.025, where x is the activity (IU/mL), and 0.025 is the conversion factor of the standard nisin solution (0.025 mg/mL = 1 IU/mL).

^b The differences of the concentration means of Nisin and lactic acid in each group are statistically significant (P < 0.05).

Figure 5. Kinetic profiles of nisin and lactic acid production by Lactococcus lactis subsp. lactis ATCC 11 454 during the SSF course.