LHCG receptor polymorphisms in PCOS patients

Figures & data

Figure 1. LHCGR rs12470652 c.872A>G/p.Asn291Ser and rs2293275 c.935G>A/ p.Ser312Asn polymorphisms – part of the LHCGR amino-acid chain and an example of A/G and G/A heterozygous sample analyzed by Sanger sequencing.

Table 1. Genotype frequency of the investigated polymorphism in patients and controls. (A) rs12470652 p.Asn291Ser (c.872A>G) (n = 99) and (B) rs2293275 p.Ser312Asn (c.935G>A) (n = 100).

	AA		AG		GG
Groups	n	%	n	%	n	%	p	MAF	P
(A) rs12470652: c.872A>G, p.Asn291Ser
Control group	37	92.5%	3	7.5%	0	0%	1.000	0.037	0.893
PCOS women	55	93.2%	4	6.8%	0	0%		0.034
	GG		AG		AA
Groups	n	%	n	%	n	%	p	MAF	P
(B) rs2293275: c.935G>A, p.Ser312Asn
Control group	21	52.5%	14	35.0%	5	12.5%	0.491	0.30	0.227
PCOS women	27	45.0%	20	33.3%	13	21.7%		0.38

Note: MAF, minor allele frequency.

Table 2. Clinical and laboratory characteristics of the PCOS patients with different rs2293275 p.Ser312Asn genotypes.

	GG (n = 27)		AG (n = 20)		AA (n = 13)
	Mean ± SD	Med	Mean ± SD	Med	Mean ± SD	Med
Age (years)	24.85 ± 5.19	24.00	26.20 ± 5.45	26.50	26.31 ± 3.59	27.00
BMI (kg/m^2)	26.79 ± 7.87	22.22	28.72 ± 7.27	27.35	30.10 ± 10.58	25.93
Waist (cm)	84.78 ± 20.57	79.00	91.95 ± 18.88	92.50	91.61 ± 19.12	95.00
Hirsutism	59.3%		70.0%		76.9%
Acne	7.4%		25%		30.8%
HDL-cholesterol (mmol/L)	1.58 ± 0.61	1.51	1.48 ± 0.42	1.53	1.49 ± 0.36	1.57
Triglycerides (mmol/L)	1.12 ± 0.62	0.86	1.45 ± 1.60	1.03	1.36 ± 1.50	0.99
Glucose (mmol/L)	5.27 ± 0.83	5.11	5.41 ± 0.93	5.30	5.39 ± 1.28	5.40
Insulin (µmol/mL)	14.71 ± 11.81	10.80	11.91 ± 5.39	10.05	14.23 ± 8.72	11.90
HOMA-IR	3.46 ± 3.28	2.33	2.80 ± 1.39	2.06	3.56 ± 2.71	2.61
Testosterone (nmol/L)	4.28 ± 1.92	4.30	4.01 ± 1.42	3.80	3.95 ± 1.51	4.00
LH (IU/L)	8.57 ± 7.85	5.85	6.32 ± 6.67	4.40	5.23 ± 3.48	4.60
FSH (IU/L)	6.05 ± 2.54	6.50	5.84 ± 1.67	5.60	5.57 ± 2.10	5.25
LH/FSH	1.62 ± 1.39	1.13	1.25 ± 1.67	0.72	1.00 ± 0.69	0.67
DHEAS (µmol/L)	9.32 ± 4.64	8.55	8.95 ± 2.18	8.60	10.85 ± 6.20	11.20
Menarche (years)	12.78 ± 1.05	13.00	13.25 ± 1.74	13.00	12.61 ± 1.80	12.00
Pregnancies	22.2%		10.0%		15.4%
Infertility*	14.8%		40.0%		7.7%

*p < 0.05.