No association between Id2 gene methylation and tetralogy of Fallot: a case-control study in China children

Figures & data

Table 1. CpG island-rich region in the promoter for specific amplification of human Id2 gene.

Site	Primer sequence	Product length (bp)	Covering CpG numbers
chr2:8680984–8681580	Forward:5’-aggaagagagTTAGGTGTTGGAATGTGATATTAGAAA-3’	597	13
	Reverse: 5’-cagtaatacgactcactatagggagaaggctCCAAACCCTACACACCTTAAAACTA-3’

Table 2. Basic characteristics and clinical data of TOF and control subjects.

Characteristics		Control	TOF	P
Cases (N)		32	31
Gender	Female	9(28%)	8(26%)	0.84
	Male	23(72%)	23(74%)
Age (m)	Mean±SD	17 ± 6.19	16.42 ± 6.25	0.71
	Minimum	8	9
	Maximum	27	26
Height (cm)	Mean±SD	83.63 ± 8.04	75.42 ± 7.02	<0.01
	Minimum	72	61
	Maximum	100	88
Body weight (kg)	Mean±SD	12.09 ± 1.96	9.47 ± 1.64	<0.01
	Minimum	8	7
	Maximum	15	13
RBC (10^12/L)	Mean±SD	4.61 ± 0.41	5.82 ± 0.94	<0.01
	Minimum	3.53	4.52
	Maximum	5.61	7.30
HGB(g/L)	Mean±SD	120.78 ± 10.07	149.32 ± 27.13	<0.01
	Minimum	105	107
	Maximum	145	208
HCT(%)	Mean±SD	36.58 ± 2.90	44.67 ± 7.18	<0.01
	Minimum	32.50	32.50
	Maximum	43.50	59.80
MCV(fl)	Mean±SD	79.24 ± 4.32	76.71 ± 9.75	0.19
	Minimum	68.20	57.55
	Maximum	92.10	97.40
MCH(pg)	Mean±SD	26.16 ± 1.64	26.02 ± 4.74	0.87
	Minimum	22.10	15.52
	Maximum	31.20	35.20
MCHC(g/L)	Mean±SD	330.06 ± 9.82	334.46 ± 28.75	0.42
	Minimum	311	246.2
	Maximum	347	387

Figure 1. Id2 gene sequence analysis indicating CpG islands.

Figure 2. Cluster analysis of 13 detectable CpG sites of the Id2 gene.

Figure 3. Methylation sequencing of Id2_CpG sites.

Table 3. Comparison of methylation levels at Id2_GpG sites.

Methylation levels		Control (n = 32)	TOF (n = 31)	χ^2	P
CpG_2	Minimum	0.00	0.00	25.86	0.58
	Maximum	0.39	0.38
CpG_3	Minimum	0.00	0.00	21.43	0.49
	Maximum	0.21	0.25
CpG_5	Minimum	0.00	0.01	11.98	0.29
	Maximum	0.09	0.17
CpG_7	Minimum	0.00	0.00	3.12	0.79
	Maximum	0.06	0.05
CpG_11.12	Minimum	0.05	0.07	6.42	0.78
	Maximum	0.16	0.15
CpG_16	Minimum	0.00	0.00	7.28	0.4
	Maximum	0.14	0.29
CpG_17	Minimum	0.02	0.01	8.28	0.31
	Maximum	0.08	0.08
CpG_18.19	Minimum	0.00	0.00	13.55	0.48
	Maximum	0.11	0.16
CpG_21.22	Minimum	0.07	0.04	9.05	0.43
	Maximum	0.14	0.14
CpG_23	Minimum	0.00	0.00	1.73	0.79
	Maximum	0.03	0.04

Figure 4. Correlation between the serum content of MCH and the methylation level at the Id2_CpG_16 site.

Figure 5. Correlation between the serum content of MCHC and the methylation level at the Id2_CpG_16 site.

Figure 6. Correlation between the serum content of MCH and the methylation level at Id2_CpG_18.19 site.

Figure 7. Correlation between the serum content of MCHC and the methylation level at Id2_CpG_18.19 site.