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Articles

Classical swine fever virus-Shimen infection upregulates SH3GLB1 expression in porcine alveolar macrophages

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Pages 93-97 | Received 17 Aug 2018, Accepted 23 Nov 2018, Published online: 12 Jan 2019

Figures & data

Figure 1. mRNA expression of SH3GLB1 during CSFV Shimen infection. (a) DGE analysis showing upregulation of SH3GLB1 with CSFV Shimen infection; (b) SH3GLB1 mRNA expression level during CSFV Shimen infection determined by qRT-PCR analysis.

Note: Statistical analysis was performed by ANOVA; **p < 0.01.

Figure 1. mRNA expression of SH3GLB1 during CSFV Shimen infection. (a) DGE analysis showing upregulation of SH3GLB1 with CSFV Shimen infection; (b) SH3GLB1 mRNA expression level during CSFV Shimen infection determined by qRT-PCR analysis.Note: Statistical analysis was performed by ANOVA; **p < 0.01.

Figure 2. Western-blotting analysis of SH3GLB1 in macrophages infected with CSFV Shimen.

Note: β-actin was used as the loading reference.

Figure 2. Western-blotting analysis of SH3GLB1 in macrophages infected with CSFV Shimen.Note: β-actin was used as the loading reference.

Figure 3. Temporal co-localization of SH3GLB1 with CSFV E2 in control cells and macrophages infected with CSFV Shimen. CSFV E2 antibody is displayed in green colour, SH3GLB1 antibody produced green fluorescence, and nuclei produced blue fluorescence.

Figure 3. Temporal co-localization of SH3GLB1 with CSFV E2 in control cells and macrophages infected with CSFV Shimen. CSFV E2 antibody is displayed in green colour, SH3GLB1 antibody produced green fluorescence, and nuclei produced blue fluorescence.

Figure 4. Role of SH3GLB1 as predicted by KEGG analysis. (a) Predicted protein–protein interactions involving SH3GLB1; (b) KEGG pathway enrichment analysis predicting SH3GLB1 involvement in several pathways, primarily, endocytosis, ErBb signalling pathway and proteoglycans in cancer.

Figure 4. Role of SH3GLB1 as predicted by KEGG analysis. (a) Predicted protein–protein interactions involving SH3GLB1; (b) KEGG pathway enrichment analysis predicting SH3GLB1 involvement in several pathways, primarily, endocytosis, ErBb signalling pathway and proteoglycans in cancer.